Naka W, Fukuda T, Ohmi T, Kanai K, Nishikawa T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
J Med Vet Mycol. 1995 Mar-Apr;33(2):141-3.
The ultrastructure of Trichophyton mentagrophytes cells stained with neutral red was investigated using electron microscopy and X-ray microanalysis. Fixatives containing molybdenum and chromium were used to prevent the outflow of neutral red. Electron-dense particles composed of metals and dye were observed exclusively in the vacuoles, which were increased in number and size, but not near the cell wall. Results indicate that neutral red passes directly through the fungal cell wall and is incorporated into the vacuoles.
使用电子显微镜和X射线微量分析研究了用中性红染色的须癣毛癣菌细胞的超微结构。使用含有钼和铬的固定剂来防止中性红流出。仅在液泡中观察到由金属和染料组成的电子致密颗粒，液泡的数量和大小增加，但在细胞壁附近未观察到。结果表明，中性红直接穿过真菌细胞壁并被纳入液泡。
