从RNA中去除DNA。

Removal of DNA from RNA.

作者信息

Rio Donald C, Ares Manuel, Hannon Gregory J, Nilsen Timothy W

出版信息

Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5443. doi: 10.1101/pdb.prot5443.

PMID:20516181

Abstract

DNA contamination of RNA preparations can present significant problems, especially for polymerase chain reaction (PCR)-based applications. No RNA extraction procedure excludes DNA entirely. Cytoplasmic RNA can be contaminated with DNA because of nuclei breakage during preparation. Moreover, TRIzol preparations do not exclude plasmids or other small DNA fragments. This article describes the reliable and effective method of eliminating DNA from RNA preparations via DNase digestion. DNase I specifically digests DNA into small oligonucleotides, leaving RNA intact.

摘要

RNA制品中的DNA污染可能会带来重大问题，尤其是对于基于聚合酶链反应（PCR）的应用。没有任何RNA提取程序能完全排除DNA。在制备过程中，由于细胞核破裂，细胞质RNA可能会被DNA污染。此外，TRIzol制品不能排除质粒或其他小DNA片段。本文介绍了一种通过DNase消化从RNA制品中去除DNA的可靠有效方法。DNase I能将DNA特异性地消化成小的寡核苷酸，而使RNA保持完整。