Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, 46202, USA.
Blood. 2010 Sep 2;116(9):1623-6. doi: 10.1182/blood-2009-08-237040. Epub 2010 Jun 1.
Gr1(+)CD11b(+) cells are characterized as myeloid-derived suppressor cells potentially involved in angiogenesis. We demonstrate that Gr1(+)CD11b(+) cells isolated from ischemic muscle in a hind-limb ischemic C57BL/6 mouse model play a role in vessel formation after ischemic injury. Gr1(dim)CD11b(+) cells, a subpopulation of Gr1(+)CD11b(+) cells, within skeletal muscle were increased in context of ischemia. Strikingly, astrocyte-plexus formed from muscle-derived Gr1(dim)CD11b(+) cells in Matrigel culture, followed by formation of isolectin and von Willebrand Factor-expressing cells, similar to that reported for angiogenesis in retina. When isolated muscle-derived Gr1(dim)CD11b(+) cells were injected into ischemic muscles, recovery of blood flow was significantly enhanced and these cells were incorporated into vessel walls. This suggests that Gr1(dim)CD11b(+) cells are recruited into ischemic regions after ischemia and may be involved in angiogenesis by their capacity to generate vascular cells.
Gr1(+)CD11b(+) 细胞被认为是髓系来源的抑制性细胞，可能参与血管生成。我们证明，从小鼠后肢缺血模型缺血肌肉中分离出的 Gr1(+)CD11b(+)细胞在缺血损伤后参与血管形成。Gr1(dim)CD11b(+) 细胞是 Gr1(+)CD11b(+)细胞的一个亚群，在缺血情况下骨骼肌中增加。引人注目的是，源自肌肉来源的 Gr1(dim)CD11b(+)细胞在 Matrigel 培养物中形成星形胶质细胞丛，随后形成异凝集素和血管性血友病因子表达细胞，类似于视网膜血管生成的情况。当分离的肌肉来源的 Gr1(dim)CD11b(+)细胞被注射到缺血肌肉中时，血流恢复明显增强，这些细胞被整合到血管壁中。这表明 Gr1(dim)CD11b(+)细胞在缺血后被募集到缺血区域，并可能通过生成血管细胞的能力参与血管生成。
