Doan Thi-Ngoc-Thanh, Prabhu Ponnandy, Kim Jin-Kwang, Ahn Yeh-Jin, Natarajan Sampath, Kang Lin-Woo, Park Geon Tae, Lim Sang-Boem, Lee Jung-Kul
Department of Advanced Technology Fusion, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):677-80. doi: 10.1107/S174430911001256X. Epub 2010 May 26.
L-Rhamnose isomerases catalyze isomerization between L-rhamnose (6-deoxy-L-mannose) and L-rhamnulose (6-deoxy-L-fructose), which is the first step in rhamnose catabolism. L-Rhamnose isomerase from Bacillus halodurans ATCC BAA-125 (BHRI) exhibits interesting characteristics such as high thermostability and selective substrate specificity. BHRI fused with an HHHHHH sequence was purified and crystallized in order to elucidate the molecular basis of its unique enzymatic properties. The crystals were grown by the hanging-drop vapour-diffusion method and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 83.2, b = 164.9, c = 92.0 A, beta = 116.0 degrees . Diffraction data were collected to 2.5 A resolution. According to a Matthews coefficient calculation, there are four monomers in the asymmetric unit with a V(M) of 3.0 A(3) Da(-1) and a solvent content of 59.3%. The initial structure of BHRI has been determined by the molecular-replacement method.
L-鼠李糖异构酶催化L-鼠李糖（6-脱氧-L-甘露糖）和L-鼠李酮糖（6-脱氧-L-果糖）之间的异构化反应，这是鼠李糖分解代谢的第一步。来自嗜碱芽孢杆菌ATCC BAA-125的L-鼠李糖异构酶（BHRI）具有一些有趣的特性，如高热稳定性和选择性底物特异性。为了阐明其独特酶学性质的分子基础，对与HHHHHH序列融合的BHRI进行了纯化和结晶。晶体通过悬滴气相扩散法生长，属于单斜空间群P2(1)，晶胞参数a = 83.2、b = 164.9、c = 92.0 Å，β = 116.0°。衍射数据收集到2.5 Å分辨率。根据马修斯系数计算，不对称单元中有四个单体，V(M)为3.0 Å3 Da-1，溶剂含量为59.3%。BHRI的初始结构已通过分子置换法确定。
