传染性支气管炎病毒非结构蛋白9的结晶及初步X射线衍射研究
Crystallization and preliminary X-ray diffraction studies of infectious bronchitis virus nonstructural protein 9.
作者信息
Ma Yanlin, Chen Cheng, Wei Lei, Yang Qingzhu, Liao Ming, Li Xuemei
机构信息
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):706-8. doi: 10.1107/S174430911001417X. Epub 2010 May 29.
Avian infectious bronchitis virus (IBV), which causes respiratory disease in infected birds, belongs to coronavirus group 3. IBV encodes 15 nonstructural proteins (nsp2-nsp16) which play crucial roles in RNA transcription and genome replication. Nonstructural protein 9 (nsp9) has been identified as a protein that is essential to viral replication because of its single-stranded RNA-binding ability. The gene segment encoding IBV nsp9 has been cloned and expressed in Escherichia coli. The protein has been crystallized and the crystals diffracted X-rays to 2.44 A resolution. They belonged to the cubic space group I432, with unit-cell parameters a = b = c = 123.4 A, alpha = beta = gamma = 90 degrees . The asymmetric unit appeared to contain one molecule, with a solvent content of 62% (V(M) = 3.26 A(3) Da(-1)).
禽传染性支气管炎病毒（IBV）可导致受感染禽类出现呼吸道疾病，属于冠状病毒第3组。IBV编码15种非结构蛋白（nsp2 - nsp16），这些蛋白在RNA转录和基因组复制中发挥关键作用。非结构蛋白9（nsp9）因其单链RNA结合能力而被确定为病毒复制所必需的蛋白。编码IBV nsp9的基因片段已在大肠杆菌中克隆并表达。该蛋白已结晶，其晶体对X射线的衍射分辨率达到2.44 Å。它们属于立方空间群I432，晶胞参数a = b = c = 123.4 Å，α = β = γ = 90°。不对称单元似乎包含一个分子，溶剂含量为62%（V(M) = 3.26 ų Da⁻¹）。
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