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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):738-43. doi: 10.1107/S174430911001626X. Epub 2010 May 29.
2
Crystal structure of 3-isopropylmalate dehydrogenase in complex with NAD(+) and a designed inhibitor.3-异丙基苹果酸脱氢酶与 NAD(+)和一种设计抑制剂复合物的晶体结构。
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3
Atomic level description of the domain closure in a dimeric enzyme: thermus thermophilus 3-isopropylmalate dehydrogenase.二聚体酶中结构域封闭的原子水平描述：嗜热栖热菌3-异丙基苹果酸脱氢酶
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4
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本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Crystal structure of 3-isopropylmalate dehydrogenase in complex with NAD(+) and a designed inhibitor.3-异丙基苹果酸脱氢酶与 NAD(+)和一种设计抑制剂复合物的晶体结构。
Bioorg Med Chem. 2009 Nov 15;17(22):7789-94. doi: 10.1016/j.bmc.2009.09.025. Epub 2009 Sep 19.
3
Rates of unfolding, rather than refolding, determine thermal stabilities of thermophilic, mesophilic, and psychrotrophic 3-isopropylmalate dehydrogenases.去折叠而非重折叠的速率决定了嗜热、嗜温和嗜冷3-异丙基苹果酸脱氢酶的热稳定性。
Biochemistry. 2007 Oct 16;46(41):11536-49. doi: 10.1021/bi700754q. Epub 2007 Sep 22.
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The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis.结核分枝杆菌中LeuB（Rv2995c）的高分辨率结构。
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5
Cryocrystallography of 3-Isopropylmalate dehydrogenase from Thermus thermophilus and its chimeric enzyme.嗜热栖热菌3-异丙基苹果酸脱氢酶及其嵌合酶的低温晶体学
Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):623-30. doi: 10.1107/S0907444995016623.
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Identification of specific interactions that drive ligand-induced closure in five enzymes with classic domain movements.鉴定在五种具有典型结构域运动的酶中驱动配体诱导的结构闭合的特定相互作用。
J Mol Biol. 2004 Jun 11;339(4):1001-21. doi: 10.1016/j.jmb.2004.04.004.
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Exploring the range of protein flexibility, from a structural proteomics perspective.从结构蛋白质组学的角度探索蛋白质灵活性的范围。
Curr Opin Chem Biol. 2004 Feb;8(1):14-9. doi: 10.1016/j.cbpa.2003.12.006.
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Purification and characterization of recombinant 3-isopropylmalate dehydrogenases from Thermus thermophilus and other microorganisms.嗜热栖热菌及其他微生物中重组3-异丙基苹果酸脱氢酶的纯化与特性分析
Methods Enzymol. 2000;324:301-22. doi: 10.1016/s0076-6879(00)24240-7.
9
Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase.镜像突变揭示了亚基间离子簇在3-异丙基苹果酸脱氢酶稳定性中的重要性。
FEBS Lett. 2000 Feb 18;468(1):48-52. doi: 10.1016/s0014-5793(00)01190-x.
10
SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.SFCHECK：一套用于评估大分子结构因子数据质量及其与原子模型一致性的统一程序。
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):191-205. doi: 10.1107/S0907444998006684. Epub 1999 Jan 1.

嗜热栖热菌异丙基苹果酸脱氢酶各种酶-底物复合物的结晶及初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of various enzyme-substrate complexes of isopropylmalate dehydrogenase from Thermus thermophilus.

作者信息

Merli Angelo, Manikandan Karuppasamy, Gráczer Eva, Schuldt Linda, Singh Rajesh Kumar, Závodszky Péter, Vas Mária, Weiss Manfred S

机构信息

Department of Biochemistry and Molecular Biology, University of Parma, Viale G. P. Usberti 23/A, 43100 Parma, Italy.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):738-43. doi: 10.1107/S174430911001626X. Epub 2010 May 29.

DOI:10.1107/S174430911001626X
PMID:20516614
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2882784/
Abstract

The Thermus thermophilus 3-isopropylmalate dehydrogenase (Tt-IPMDH) enzyme catalyses the penultimate step of the leucine-biosynthesis pathway. It converts (2R,3S)-3-isopropylmalate to (2S)-2-isopropyl-3-oxosuccinate in the presence of divalent Mg(2+) or Mn(2+) and with the help of NAD(+). In order to elucidate the detailed structural and functional mode of the enzymatic reaction, crystals of Tt-IPMDH were grown in the presence of various combinations of substrate and/or cofactors. Here, the crystallization, data collection and preliminary crystallographic analyses of six such complexes are reported.

摘要

嗜热栖热菌3-异丙基苹果酸脱氢酶（Tt-IPMDH）催化亮氨酸生物合成途径的倒数第二步反应。在二价镁离子（Mg(2+)）或锰离子（Mn(2+)）存在的情况下，借助烟酰胺腺嘌呤二核苷酸（NAD(+)），它将（2R,3S）-3-异丙基苹果酸转化为（2S）-2-异丙基-3-氧代琥珀酸。为了阐明酶促反应的详细结构和功能模式，在底物和/或辅因子的各种组合存在的情况下培养了Tt-IPMDH晶体。在此，报道了六种此类复合物的结晶、数据收集和初步晶体学分析。