Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, #EA-03-12, Singapore 129789.
Opt Lett. 2010 Jun 1;35(11):1804-6. doi: 10.1364/OL.35.001804.
Focal modulation microscopy is an emerging fluorescence microscopy technique for in vivo imaging of thick biological tissues. Here, we present a theoretical study to assess its performance. The scalar diffraction theory is combined with Monte Carlo simulation to evaluate the signal-to-background ratio at various depths. The performance of confocal microscopy with a similar optical setup is also evaluated for comparison.
焦点调制显微镜是一种新兴的荧光显微镜技术，用于厚生物组织的体内成像。在这里，我们进行了一项理论研究来评估其性能。我们结合标量衍射理论和蒙特卡罗模拟来评估在不同深度处的信号-背景比。我们还评估了具有类似光学设置的共焦显微镜的性能，以进行比较。
