Dipartimento di Biologia cellulare e dello Sviluppo, Università di Palermo, 90128 Palermo, Italy.
J Agric Food Chem. 2010 Jul 14;58(13):7565-71. doi: 10.1021/jf100434e.
We investigated, using an organ bath technique, the effects of a hydrophilic extract from Opuntia ficus indica fruit pulp (cactus fruit extract, CFE) on the motility of mouse ileum, and researched the extract component(s) responsible for the observed responses. CFE (10-320 mg of fresh fruit pulp equivalents/mL of organ bath) reduced dose-dependently the spontaneous contractions. This effect was unaffected by tetrodotoxin, a neuronal blocker, N(omega)-nitro-l-arginine methyl ester, a nitric oxide synthase blocker, tetraethylammonium, a potassium channel blocker, or atropine, a muscarinic receptor antagonist. CFE also reduced the contractions evoked by carbachol, without affecting the contractions evoked by high extracellular potassium. Indicaxanthin, but not ascorbic acid, assayed at concentrations comparable with their content in CFE, mimicked the CFE effects. The data show that CFE is able to exert direct antispasmodic effects on the intestinal motility. The CFE inhibitory effects do not involve potassium channels or voltage-dependent calcium channels but rather pathways of calcium intracellular release. The fruit pigment indicaxanthin appears to be the main component responsible for the CFE-induced effects.
我们采用器官浴技术研究了仙人掌果实（仙人掌果提取物，CFE）的亲水提取物对小鼠回肠运动的影响，并研究了负责观察到的反应的提取物成分。CFE（器官浴中每毫升 10-320 毫克鲜果肉当量）剂量依赖性地减少自发性收缩。这种作用不受神经元阻断剂河豚毒素、一氧化氮合酶阻断剂 N（omega）-硝基-L-精氨酸甲酯、钾通道阻断剂四乙铵或毒蕈碱受体拮抗剂阿托品的影响。CFE 还减少了乙酰胆碱引起的收缩，而不影响高细胞外钾引起的收缩。Indicaxanthin（而不是抗坏血酸）在与 CFE 中含量相当的浓度下模拟了 CFE 的作用。数据表明，CFE 能够对肠道运动产生直接的解痉作用。CFE 的抑制作用不涉及钾通道或电压依赖性钙通道，而是涉及细胞内钙释放途径。水果色素 indicaxanthin 似乎是负责 CFE 诱导作用的主要成分。
