Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 28, 90123 Palermo, Italy.
Oxid Med Cell Longev. 2019 Feb 18;2019:3457846. doi: 10.1155/2019/3457846. eCollection 2019.
Oxidized low-density lipoproteins (oxLDL) play a pivotal role in the etiopathogenesis of atherosclerosis through the activation of inflammatory signaling events eventually leading to endothelial dysfunction and senescence. In the present work, we investigated the effects of indicaxanthin, a bioavailable, redox-modulating phytochemical from fruits, with anti-inflammatory activity, against oxLDL-induced endothelial dysfunction. Human umbilical vein cord cells (HUVEC) were stimulated with human oxLDL, and the effects of indicaxanthin were evaluated in a range between 5 and 20 M, consistent with its plasma level after a fruit meal (7 M). Pretreatment with indicaxanthin significantly and concentration-dependently inhibited oxLDL-induced cytotoxicity; ICAM-1, VCAM-1, and ELAM-1 increase; and ABC-A1 decrease of both protein and mRNA levels. From a mechanistic perspective, we also provided evidence that the protective effects of indicaxanthin were redox-dependent and related to the pigment efficacy to inhibit NF-B transcriptional activity. In conclusion, here we demonstrate indicaxanthin as a novel, dietary phytochemical, able to exert significant protective vascular effects , at nutritionally relevant concentrations.
氧化型低密度脂蛋白（oxLDL）通过激活炎症信号事件在动脉粥样硬化的发病机制中起关键作用，最终导致内皮功能障碍和衰老。在本工作中，我们研究了 indicaxanthin 的作用，它是一种来自水果的具有抗炎活性的生物可利用的氧化还原调节植物化学物质，针对 oxLDL 诱导的内皮功能障碍。用人 oxLDL 刺激人脐静脉内皮细胞（HUVEC），并在 5 到 20μM 的范围内评估 indicaxanthin 的作用，这与水果餐后其在血浆中的水平（7μM）一致。 indicaxanthin 的预处理显著且浓度依赖性地抑制 oxLDL 诱导的细胞毒性；增加 ICAM-1、VCAM-1 和 ELAM-1 的增加；以及 ABCA1 的蛋白和 mRNA 水平降低。从机制角度来看，我们还提供了证据表明，indicaxanthin 的保护作用是依赖于氧化还原的，与色素抑制 NF-B 转录活性的功效有关。总之，我们证明了 indicaxanthin 是一种新型的饮食植物化学物质，能够在营养相关浓度下发挥显著的保护血管作用。
