Department of Microbiology, Department of Biochemistry, and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Am J Trop Med Hyg. 2010 Jun;82(6):1102-5. doi: 10.4269/ajtmh.2010.09-0567.
Profiles of Burkholderia pseudomallei persistence and antibodies in blood, spleen, liver, and lungs of infected BALB/c mice were investigated. Animals were infected intraperitoneally with low (6 colony-forming units) or high (230 colony-forming units) doses of B. pseudomallei. In the high-dose infected group, bacteria were found by culture in 100% of blood, liver, spleen and lung samples at 24 and 48 hours after infections; blood samples were 100% positive by polymerase chain reaction after 60 hours. Antibody responses in the high-dose infected group were low. These responses were detected in the low-dose infected group after 5 days, peaked at 7-14 days, and showed persistence until 28 days post-infection. Bacterial loads and antibody profiles varied according to the level of bacterial infections. This kinetic study, although in animals, provides crucial knowledge that might be useful for the development of a sensitive and specific diagnostic assay for patients with melioidosis.
本研究调查了感染 BALB/c 小鼠血液、脾脏、肝脏和肺部中伯克霍尔德氏菌（Burkholderia pseudomallei）持久性和抗体的特征。动物通过腹腔内感染低（6 个菌落形成单位）或高（230 个菌落形成单位）剂量的伯克霍尔德氏菌。在高剂量感染组中，在感染后 24 和 48 小时，细菌通过培养在 100%的血液、肝脏、脾脏和肺部样本中被发现；在 60 小时后，血液样本通过聚合酶链反应（PCR）100%呈阳性。高剂量感染组的抗体反应较低。在低剂量感染组中，这些反应在 5 天后被检测到，在 7-14 天达到峰值，并在感染后 28 天持续存在。细菌载量和抗体谱根据细菌感染水平而变化。虽然这项动物研究提供了关键的知识，可能对开发针对类鼻疽病患者的敏感和特异的诊断检测具有重要意义。
