Center for Predictive Medicine, Department of Microbiology and Immunology, University of Louisville Louisville, KY, USA.
Front Microbiol. 2010 Dec 29;1:141. doi: 10.3389/fmicb.2010.00141. eCollection 2010.
Burkholderia pseudomallei is the Gram-negative bacterial pathogen responsible for the disease melioidosis. B. pseudomallei establishes disease in susceptible individuals through multiple routes of infection, all of which may proceed to a septicemic disease associated with a high mortality rate. B. pseudomallei opportunistically infects humans and a wide range of animals directly from the environment, and modeling of experimental melioidosis has been conducted in numerous biologically relevant models including mammalian and invertebrate hosts. This review seeks to summarize published findings related to established animal models of melioidosis, with an aim to compare and contrast the virulence of B. pseudomallei in these models. The effect of the route of delivery on disease is also discussed for intravenous, intraperitoneal, subcutaneous, intranasal, aerosol, oral, and intratracheal infection methodologies, with a particular focus on how they relate to modeling clinical melioidosis. The importance of the translational validity of the animal models used in B. pseudomallei research is highlighted as these studies have become increasingly therapeutic in nature.
类鼻疽伯克霍尔德菌是一种革兰氏阴性细菌病原体，可导致类鼻疽病。类鼻疽伯克霍尔德菌通过多种感染途径在易感个体中引发疾病，所有这些途径都可能导致败血性疾病，死亡率很高。类鼻疽伯克霍尔德菌机会性地直接从环境中感染人类和广泛的动物，并且已经在许多生物学相关模型中进行了实验性类鼻疽病的建模，包括哺乳动物和无脊椎动物宿主。本综述旨在总结已发表的关于类鼻疽病既定动物模型的研究结果，旨在比较和对比这些模型中类鼻疽伯克霍尔德菌的毒力。还讨论了不同给药途径对疾病的影响，包括静脉内、腹腔内、皮下、鼻内、气溶胶、口服和气管内感染方法，特别关注它们与临床类鼻疽病建模的关系。由于这些研究在性质上越来越具有治疗性，因此强调了在类鼻疽伯克霍尔德菌研究中使用的动物模型的转化有效性的重要性。
