Kerns J M, Fakhouri A J, Weinrib H P, Freeman J A
Department of Anatomy, Rush-Presbyterian-St Luke's Medical Center, Chicago, IL 60612.
Neuroscience. 1991;40(1):93-107. doi: 10.1016/0306-4522(91)90177-p.
This study examines the effect of applied d.c. electric fields on nerve regeneration following injury to the rat sciatic nerve using the circularly vibrating probe and electron microscopy. The transected and treated nerve which received a d.c. electrical stimulator (0.6 mu A) was compared with untreated transected and crushed nerves. At one week postoperative, the probe was used to measure in vivo the current density along the nerve length. All nerves studied had a proximal peak at the lesion site and a second peak at varying distal locations: crushed/untreated (13.3 mm), transected/untreated (9.7 mm) and transected/treated (16.3 mm). A significant difference (69%) between the distal peak distances in the two transection groups suggests that the electrical treatment enhanced the progress of nerve regeneration. There were no significant differences between the mean peak amplitudes (1.6-2.2 mu A/cm2). Applied verapamil reduced the peaks, suggesting they are associated in part with a calcium-dependent current. Electron microscopy at selected nerve regions indicated that the peaks correspond to regenerating axonal growth cones. The results suggest the potential clinical application of d.c. electric fields in the treatment of nerve injuries.
本研究使用环形振动探针和电子显微镜，研究了施加直流电电场对大鼠坐骨神经损伤后神经再生的影响。将接受直流电刺激器（0.6微安）的横断并处理过的神经与未处理的横断和挤压神经进行比较。术后一周，使用探针在体内测量沿神经长度的电流密度。所有研究的神经在损伤部位有一个近端峰值，在不同的远端位置有第二个峰值：挤压/未处理（13.3毫米）、横断/未处理（9.7毫米）和横断/处理（16.3毫米）。两个横断组远端峰值距离之间存在显著差异（69%），表明电处理促进了神经再生进程。平均峰值幅度（1.6 - 2.2微安/平方厘米）之间无显著差异。应用维拉帕米降低了峰值，表明它们部分与钙依赖性电流有关。选定神经区域的电子显微镜检查表明，峰值对应于再生轴突生长锥。结果表明直流电电场在治疗神经损伤方面具有潜在的临床应用价值。
