Department of Oriental Gynecology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
J Med Food. 2010 Jun;13(3):564-71. doi: 10.1089/jmf.2009.1252.
Cyperi rhizoma, the rhizome of Cyperus rotundus L. (Family Cyperaceae), is a well-known functional food and traditional herbal medicine in Korea. It has been reported that Cyperi rhizoma has antioxidant and free radical scavenging activities that play a major role in protection of neurodegenerative disorders, such as Parkinson's disease (PD). In the present study, the neuroprotective effects of a water extract of Cyperi rhizoma (CRE) against 6-hydroxydopamine (6-OHDA)-induced neuronal damage were evaluated in an experimental model of PD. In PC12 cells, CRE showed a significant protective effect on cell viability at 50 and 100 microg/mL. CRE inhibited generation of reactive oxygen species and nitric oxide, reduction of mitochondrial membrane potential, and caspase-3 activity, which were induced by 6-OHDA. CRE also showed a significant protective effect against damage to dopaminergic neurons in primary mesencephalic culture. These results suggest that CRE has neuroprotective effects against 6-OHDA-induced toxicity through antioxidant and anti-apoptotic activities in an in vitro PD model.
香附，是莎草科莎草属植物莎草（Cyperus rotundus L.）的根茎，是韩国广受欢迎的功能性食品和传统草药。有报道称，香附具有抗氧化和清除自由基的活性，在保护神经退行性疾病（如帕金森病）方面发挥着重要作用。在本研究中，评估了香附水提物（CRE）对帕金森病实验模型中 6-羟多巴胺（6-OHDA）诱导的神经元损伤的神经保护作用。在 PC12 细胞中，CRE 在 50 和 100μg/mL 时对细胞活力表现出显著的保护作用。CRE 抑制了由 6-OHDA 诱导的活性氧和一氧化氮的产生、线粒体膜电位的降低以及半胱天冬酶-3 活性。CRE 对原代中脑培养物中多巴胺能神经元的损伤也表现出显著的保护作用。这些结果表明，CRE 通过在体外 PD 模型中的抗氧化和抗细胞凋亡活性对 6-OHDA 诱导的毒性具有神经保护作用。
