Jia Hongmei, Liu Yang, Yu Meng, Shang Hai, Zhang Hongwu, Ma Liyan, Zhang Tao, Zou Zhongmei
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Metabolites. 2019 Oct 23;9(11):244. doi: 10.3390/metabo9110244.
Cyperi Rhizoma (CR) is a well-known functional food and traditional herbal medicine in Asian countries for the treatment of menstrual or emotional disturbances in women. Recent studies have shown the pharmacological effects of CR on neuronal diseases, such as Parkinson's disease (PD) and depression. Thus, the neuroprotective effect of CR might play a vital role in exerting its effect. Here, corticosterone-induced PC12 cells were applied to screen the active fraction of CR and evaluate its neuroprotective effect. The results indicated that the fraction containing medium-polarity chemical constituents (CR-50E) displayed the best protection effect. CR-50E could increase the cell viability and reduce cell apoptosis through inhibiting oxidative stress and decreasing the lactate dehydrogenase LDH release induced by corticosterone. Further, the mechanism of action was explored by cell metabolomics. The result showed CR-50E mediated the sphingolipids metabolism of corticosterone-induced PC12 cells, which suggested inhibition of Ca overloading may involve the protection of CR-50E against cell damage. The expression levels of three key proteins in calcium transport, including phospholipase A2 (PLA2), calcium/calmodulin independent protein kinase II (CaMK II), and caspase-3, confirmed the above result by Western blot. The findings suggest that CR-50E can suppress the disequilibrium of calcium homeostasis-mediated apoptosis by improving the abnormal sphingolipids metabolism as well as remedying the damage of the cell membrane.
香附是亚洲国家一种著名的功能性食品和传统草药，用于治疗女性的月经紊乱或情绪障碍。最近的研究表明香附对神经疾病具有药理作用，如帕金森病（PD）和抑郁症。因此，香附的神经保护作用可能在其发挥作用中起着至关重要的作用。在此，应用皮质酮诱导的PC12细胞来筛选香附的活性成分并评估其神经保护作用。结果表明，含有中等极性化学成分的组分（CR-50E）显示出最佳的保护效果。CR-50E可以通过抑制氧化应激和减少皮质酮诱导的乳酸脱氢酶（LDH）释放来提高细胞活力并减少细胞凋亡。此外，通过细胞代谢组学探索其作用机制。结果表明，CR-50E介导了皮质酮诱导的PC12细胞的鞘脂代谢，这表明抑制钙超载可能参与了CR-50E对细胞损伤的保护作用。通过蛋白质免疫印迹法检测钙转运中三种关键蛋白，即磷脂酶A2（PLA2）、钙/钙调蛋白依赖性蛋白激酶II（CaMK II）和半胱天冬酶-3的表达水平，证实了上述结果。研究结果表明，CR-50E可以通过改善异常的鞘脂代谢以及修复细胞膜损伤来抑制钙稳态失衡介导的细胞凋亡。
