Eager K B
Bristol-Myers Squibb Pharmaceutical Research Institute, Department of Molecular Biology, Princeton, New Jersey 08543-4000.
Oncogene. 1991 May;6(5):819-24.
Monoclonal antibodies specific for the human trk protooncogene product, a tyrosine kinase receptor, have been produced from mice in which tumors were generated by injection of stably transfected NIH3T3 cells expressing the human trk proto-oncogene product. The panel of eleven antibodies are reactive in ELISA, immunostaining and immunoprecipitation. These antibodies bind to the extracellular domain of the human trk proto-oncogene product and demonstrate no cross-reactivity to the trk oncogene or murine trkB gene products. The antibodies are equally effective in recognizing human proto-trk when expressed by transfected human or mouse cell lines. These antibodies either bind to the carbohydrate moieties or are dependent upon the conformational structure created by the extensive glycosylations. The localization and nature of the epitopes recognized by these monoclonal antibodies were defined by immunoprecipitation analyses using several different trk oncoproteins. Potential applications of these antibodies are discussed.
针对人trk原癌基因产物（一种酪氨酸激酶受体）的单克隆抗体，是由小鼠产生的，在这些小鼠中，通过注射表达人trk原癌基因产物的稳定转染NIH3T3细胞产生肿瘤。这11种抗体在酶联免疫吸附测定（ELISA）、免疫染色和免疫沉淀中具有反应活性。这些抗体与人trk原癌基因产物的细胞外结构域结合，对trk癌基因或小鼠trkB基因产物无交叉反应。当由转染的人或小鼠细胞系表达时，这些抗体在识别人类原trk方面同样有效。这些抗体要么与碳水化合物部分结合，要么依赖于广泛糖基化产生的构象结构。通过使用几种不同的trk癌蛋白进行免疫沉淀分析，确定了这些单克隆抗体识别的表位的定位和性质。讨论了这些抗体的潜在应用。
