McKenzie S J, Marks P J, Lam T, Morgan J, Panicali D L, Trimpe K L, Carney W P
Applied bioTechnology, Cambridge, Massachusetts 02142.
Oncogene. 1989 May;4(5):543-8.
A series of monoclonal antibodies specific for the extracellular domain of the human neu gene product (p185) have been produced. The generation of these monoclonal antibodies, and their biochemical and immunological characterization is described. The immunization protocol utilized a series of injections of NIH3T3 cells, cyclophosphamide, and a neu transfected NIH3T3 cell line (designated 18-3-7) which expressed the full length human neu-encoded protein. This immunization regimen induced an immune response to the extracellular portion of p185 on the 18-3-7 cells. A panel of ten hybridomas were identified which secreted monoclonal antibodies with a variety of epitope specificities, and reacted with p185 in a number of different experimental formats. As the neu gene product has been associated with human breast cancers, a series of monoclonal antibodies such as these could prove useful in the diagnosis, prognosis and/or treatment of these human malignancies.
已制备出一系列针对人类neu基因产物（p185）细胞外结构域的单克隆抗体。本文描述了这些单克隆抗体的产生过程及其生化和免疫学特性。免疫方案采用了一系列注射NIH3T3细胞、环磷酰胺以及一种表达全长人类neu编码蛋白的neu转染NIH3T3细胞系（命名为18 - 3 - 7）。这种免疫方案诱导了针对18 - 3 - 7细胞上p185细胞外部分的免疫反应。鉴定出一组十个杂交瘤，它们分泌具有多种表位特异性的单克隆抗体，并能在多种不同实验形式中与p185发生反应。由于neu基因产物与人类乳腺癌相关，这类单克隆抗体系列可能在这些人类恶性肿瘤的诊断、预后评估和/或治疗中发挥作用。
