Ikegaki N, Kennett R H
Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia 19104.
Oncogene. 1990 Mar;5(3):397-403.
The myc family proteins were used to produce monoclonal antibodies with defined specificities. The pattern of mosaic homology among the myc family proteins facilitated the efficient identification of monoclonal antibodies specific to myc homology box sequences. Sequential epitopes for pan-myc reactive monoclonal antibodies produced against N-myc/c-myc fusion protein were further defined by use of truncated myc proteins made in E. coli and synthetic oligopeptides corresponding to myc box sequences. One class of antibodies was found to be specific to the first myc box sequence, whereas the other was found to be reactive with the third myc box sequence. Further development of anti-myc monoclonal antibodies, especially those antibodies specific to each myc box sequence, would be likely to facilitate analysis of the possible biological functions of the myc proteins in vivo and in vitro.
利用myc家族蛋白制备具有明确特异性的单克隆抗体。myc家族蛋白之间的镶嵌同源模式有助于高效鉴定针对myc同源框序列的单克隆抗体。通过使用在大肠杆菌中制备的截短型myc蛋白以及与myc框序列对应的合成寡肽，进一步确定了针对N-myc/c-myc融合蛋白产生的泛myc反应性单克隆抗体的连续表位。发现一类抗体对第一个myc框序列具有特异性，而另一类抗体则与第三个myc框序列反应。抗myc单克隆抗体的进一步开发，尤其是那些对每个myc框序列具有特异性的抗体，可能会有助于分析myc蛋白在体内和体外可能的生物学功能。
