Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan; T, Nakano--Research and Development Department, Bean Stalk Snow Co, Ltd, Kawagoe, Japan.
Allergy Asthma Clin Immunol. 2007 Mar 15;3(1):1-9. doi: 10.1186/1710-1492-3-1-1.
: Chymotrypsin- or trypsin V- (a mixture of trypsin and chymotrypsin) digested beta-lactoglobulin (BLG) peptides were prepared and were confirmed to have much less immunoglobulin (lg)G and lgE reactivity compared with intact BLG by IgG inhibition enzymelinked immunosorbent assay and IgE dot blotting. The lymphocyte responses to intact BLG and these peptides were examined using peripheral blood mononuclear cells (PBMCs) from 10 patients with cow's milk allergy. The PBMCs from most patients had lower lymphocyte responses to chymotrypsin- and trypsin V-digested BLG peptides than those to intact BLG. However, PBMCs from one and two patients retained significant proliferative responses to both peptides and to only the former peptide, respectively. Interferon-c production stimulated by chymotrypsin-digested peptides was still detectable in all five patients tested. Chymotrypsindigested BLG reduced lgE reactivity but still induced some lymphocyte responses.
糜蛋白酶或胰蛋白酶 V（胰蛋白酶和糜蛋白酶的混合物）消化的β-乳球蛋白 (BLG) 肽被制备出来，并通过 IgG 抑制酶联免疫吸附试验和 IgE 点印迹法证实与完整的 BLG 相比，其免疫球蛋白 (lgG) 和 lgE 反应性要低得多。使用来自 10 名牛奶过敏患者的外周血单核细胞 (PBMC) 检查了对完整 BLG 和这些肽的淋巴细胞反应。大多数患者的 PBMC 对糜蛋白酶和胰蛋白酶 V 消化的 BLG 肽的淋巴细胞反应低于对完整 BLG 的反应。然而，一名和两名患者的 PBMC 分别对两种肽和仅前一种肽保留了显著的增殖反应。在所有测试的五名患者中，仍然可以检测到由糜蛋白酶消化的肽刺激产生的干扰素-c。糜蛋白酶消化的 BLG 降低了 lgE 反应性，但仍诱导了一些淋巴细胞反应。
