保守剪接因子 SUA 控制拟南芥发育调节剂 ABI3 的可变剪接。
The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.
机构信息
Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
出版信息
Plant Cell. 2010 Jun;22(6):1936-46. doi: 10.1105/tpc.110.074674. Epub 2010 Jun 4.
Abstract
ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3-alpha and ABI3-beta, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-beta transcript accumulates at the end of seed maturation. The two ABI3 transcripts differ by the presence of a cryptic intron in ABI3-alpha, which is spliced out in ABI3-beta. The suppressor of abi3-5 (sua) mutant consistently restores wild-type seed features in the frameshift mutant abi3-5 but does not suppress other abi3 mutant alleles. SUA is a conserved splicing factor, homologous to the human protein RBM5, and reduces splicing of the cryptic ABI3 intron, leading to a decrease in ABI3-beta transcript. In the abi3-5 mutant, ABI3-beta codes for a functional ABI3 protein due to frameshift restoration.
摘要
脱落酸不敏感 3 型（ABI3）是拟南芥种子成熟的主要调控因子。我们检测到两种 ABI3 转录本，ABI3-α和 ABI3-β，分别编码全长和截短的蛋白。ABI3 的可变剪接受到发育调控，ABI3-β 转录本在种子成熟末期积累。两个 ABI3 转录本的区别在于 ABI3-α 中存在一个隐秘内含子，该内含子在 ABI3-β 中被剪接出来。ABI3-5 的抑制子（sua）突变体在移码突变体 abi3-5 中始终恢复野生型种子特征，但不抑制其他 abi3 突变等位基因。SUA 是一种保守的剪接因子，与人类蛋白 RBM5 同源，可减少隐秘的 ABI3 内含子的剪接，导致 ABI3-β 转录本减少。在 abi3-5 突变体中，ABI3-β 由于移码恢复而编码具有功能的 ABI3 蛋白。
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