Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore.
Nat Genet. 2010 Jul;42(7):631-4. doi: 10.1038/ng.600. Epub 2010 Jun 6.
Detection of new genomic control elements is critical in understanding transcriptional regulatory networks in their entirety. We studied the genome-wide binding locations of three key regulatory proteins (POU5F1, also known as OCT4; NANOG; and CTCF) in human and mouse embryonic stem cells. In contrast to CTCF, we found that the binding profiles of OCT4 and NANOG are markedly different, with only approximately 5% of the regions being homologously occupied. We show that transposable elements contributed up to 25% of the bound sites in humans and mice and have wired new genes into the core regulatory network of embryonic stem cells. These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells.
检测新的基因组调控元件对于全面了解转录调控网络至关重要。我们研究了三个关键调控蛋白（POU5F1，也称为 OCT4；NANOG；和 CTCF）在人类和小鼠胚胎干细胞中的全基因组结合位置。与 CTCF 不同，我们发现 OCT4 和 NANOG 的结合图谱明显不同，只有大约 5%的区域被同源占据。我们表明，转座元件在人类和小鼠中贡献了高达 25%的结合位点，并将新基因连接到胚胎干细胞的核心调控网络中。这些数据表明，物种特异性转座元件已大大改变了多能干细胞的转录电路。
