Hotter G, Catafau J R, Artigot M, Bulbena O, Pi F, Saenz A, Cruz L F, Gelpi E
Department of Neurochemistry (Eicosanoid Branch), Barcelona, Spain.
Prostaglandins. 1991 Jun;41(6):529-36. doi: 10.1016/0090-6980(91)90059-o.
Significant increases of TXB2 and PGE2 are reported to occur in pancreas transplantation. These increases are prevented with scavengers of oxygen-free radicals. In this communication, we report on changes of prostacyclin metabolites such as tissue 6-keto prostaglandin F1 alpha and urinary 2,3-dinor 6-keto prostaglandin F1 alpha in rats subjected to pancreas transplantation after different periods of organ cold preservation ischemia as well as the effect of superoxide dismutase (SOD) on these changes. For this purpose, male Lewis rats were classified as follows: Group I, Control; Group II, syngenic pancreas transplantation after 15 min of organ preservation in Collins solution at 4 degrees C; Group III, same as II but with 12 hours of organ preservation; Group IV, same as III, but with SOD pretreatment. Results have shown significant posttransplantation increases of both tissue 6-keto PGF1 alpha and urinary 2, 3 dinor 6-keto PGF1 alpha, the latter being a useful marker to evaluate systemic prostacyclin (PGI2) production by rat pancreas. This effect was prevented when the organ had been exposed to SOD during the period of cold preservation ischemia. These results confirm the implication of oxygen-free radicals (OFR) in the ischemia-reperfusion process associated to rat pancreas transplantation leading to enhanced arachidonic acid metabolism.
据报道，胰腺移植时TXB2和PGE2会显著增加。使用自由基清除剂可阻止这些增加。在本通讯中，我们报告了在不同时间段的器官冷保存缺血后接受胰腺移植的大鼠中前列环素代谢产物的变化，如组织6-酮前列腺素F1α和尿2,3-二去甲-6-酮前列腺素F1α，以及超氧化物歧化酶（SOD）对这些变化的影响。为此，将雄性Lewis大鼠分为以下几组：第一组，对照组；第二组，在4℃的柯林斯溶液中保存15分钟后进行同基因胰腺移植；第三组，与第二组相同，但器官保存12小时；第四组，与第三组相同，但进行SOD预处理。结果显示，移植后组织6-酮PGF1α和尿2,3-二去甲-6-酮PGF1α均显著增加，后者是评估大鼠胰腺产生全身前列环素（PGI2）的有用标志物。当器官在冷保存缺血期间暴露于SOD时，这种作用被阻止。这些结果证实了自由基（OFR）在与大鼠胰腺移植相关的缺血再灌注过程中的作用，导致花生四烯酸代谢增强。
