Hotter G, Leon O S, Roselló-Catafau J, López-Boado M A, Parellada P P, Henriques R D, Fernández-Cruz L, Gelpí E
Department of Surgery, Faculty of Medicine, University of Barcelona, Spain.
Transplantation. 1991 May;51(5):987-90. doi: 10.1097/00007890-199105000-00011.
Free radical species have been implicated as important agents in ischemia-reperfusion injury associated to transplantation procedures. This study was carried out to investigate the possible relationship between phospholipase A2 activity (PLA2), lipoperoxidation, and the changes in arachidonic acid metabolism during ischemia reperfusion injury in pancreas transplantation, as well as the effect of a free radical scavenger such as superoxide dismutase on these changes. For this purpose male Lewis rat groups (n = 7) were classified as follows: group I--control; group II--syngenic pancreas transplantation after 15 min preservation in Collins solution at 4 degrees C; group III--syngenic pancreas transplantation after 18 hr preservation in the same conditions; group IV--same as III but with administration of SOD (i.v.) immediately before revascularization in the recipient rat. The results indicate that significant increases in PLA2 activity and lipoperoxide levels occur concomitantly with an increase of thromboxane B2 (TXB2) and 6-keto prostaglandin F1 alpha (6-keto PGF1 alpha) in pancreatic tissue after pancreas transplantation. The counteracting effect of a free radical scavenger such as SOD supports the role of oxygen free radicals (OFR) mediating activation of PLA2 and subsequent formation of eicosanoids in pancreas transplantation.
自由基被认为是与移植手术相关的缺血再灌注损伤中的重要因素。本研究旨在探讨胰腺移植缺血再灌注损伤过程中磷脂酶A2活性（PLA2）、脂质过氧化与花生四烯酸代谢变化之间的可能关系，以及超氧化物歧化酶等自由基清除剂对这些变化的影响。为此，将雄性Lewis大鼠分为以下几组（n = 7）：第一组为对照组；第二组为在4℃下于柯林斯溶液中保存15分钟后进行同基因胰腺移植；第三组为在相同条件下保存18小时后进行同基因胰腺移植；第四组与第三组相同，但在受体大鼠血管再通前立即静脉注射超氧化物歧化酶（SOD）。结果表明，胰腺移植后胰腺组织中PLA2活性和脂质过氧化物水平显著升高，同时血栓素B2（TXB2）和6-酮前列腺素F1α（6-酮PGF1α）增加。超氧化物歧化酶等自由基清除剂的抵消作用支持了氧自由基（OFR）在胰腺移植中介导PLA2激活和随后类花生酸形成的作用。
