Department of Polymer Science and Engineering, CAS Key Laboratory of Soft Matter Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Biomacromolecules. 2010 Jul 12;11(7):1840-6. doi: 10.1021/bm100307d.
A water-soluble, biodegradable and fluorescent hyperbranched poly(amidoamine) with mannose groups on their surface (M-HPAMAM) has been successfully prepared, and the synthetic strategy includes Michael addition polymerization of diacrylamide with 1-(2-aminoethyl)piperazine and, subsequently, surface modification with mannosamine. The photoluminescence of M-HPAMAM was enhanced significantly due to the surface mannose groups. Incubation of E. coli with M-HPAMAMs yielded brightly fluorescent bacteria clusters, but the fluorescent intensity of the aqueous solution lowered. This indicates that the M-HPAMAMs have strong affinity with bacteria due to their polyvalent interactions. Based on the size and the amount of bacteria clusters formed, the bacteria with the concentrations higher than 10(2) cfu/mL can be detected.
一种水溶性、可生物降解的荧光超支化聚（酰胺-胺），其表面具有甘露糖基团（M-HPAMAM）已成功制备，其合成策略包括二丙烯酰胺与 1-（2-氨乙基）哌嗪的迈克尔加成聚合，随后用甘露糖胺进行表面修饰。由于表面甘露糖基团，M-HPAMAM 的光致发光显著增强。将大肠杆菌与 M-HPAMAMs 孵育后，得到荧光强度显著增强的细菌团簇，但水溶液的荧光强度降低。这表明由于多价相互作用，M-HPAMAMs 与细菌具有很强的亲和力。基于形成的细菌团簇的大小和数量，可以检测到浓度高于 10(2) cfu/mL 的细菌。
