Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture, Beijing 100193, China.
Molecules. 2018 Aug 16;23(8):2056. doi: 10.3390/molecules23082056.
A mannose-functionalized poly (-phenylene ethynylene) was rationally designed to achieve selective detection of bacteria. The polymer was constructed as a signaling unit and was modified by attaching aminoethyl mannose using the carboxylic acid group at the end of the linker. Incubation of with the polymer yielded fluorescent bacteria aggregates through polyvalent interactions. The utility of the mannose functionalized polymer to detect expressing functional FimH mannose-specific lectin on their surface was also demonstrated. The sugar units displayed on the surface of the polymer retained their functional ability to interact with mannose-binding lectin. To determine the optimum binding time, we measured the fluorescence intensity of the polymer-bacteria suspension at intervals. Our results showed that binding in this system will reach an optimum level within 30 min of incubation. The polymer's affinity for bacteria has been demonstrated and bacteria with a concentration of 10³ CFU mL can be detected by this system.
一种甘露糖功能化的聚（亚苯基乙炔）被合理设计用于选择性检测细菌。该聚合物被构建为信号单元，并通过在连接子末端的羧酸基团上附着氨乙基甘露糖进行修饰。与聚合物孵育会通过多价相互作用产生荧光细菌聚集物。还证明了甘露糖功能化聚合物在检测表面表达功能性 FimH 甘露糖特异性凝集素的细菌中的用途。聚合物表面上显示的糖单位保留了与甘露糖结合凝集素相互作用的功能能力。为了确定最佳结合时间，我们在不同时间间隔测量聚合物-细菌悬浮液的荧光强度。我们的结果表明，在 30 分钟的孵育时间内，该体系中的结合将达到最佳水平。该聚合物对细菌的亲和力已得到证明，该系统可以检测浓度为 10³ CFU mL 的细菌。
