Department of Chemistry, University of Tennessee, 618 Buehler Hall, Knoxville, Tennessee 37996-1600, USA.
Biochemistry. 2010 Jul 13;49(27):5621-3. doi: 10.1021/bi1001163.
Extracellular autoinducer concentrations in cultures of Vibrio harveyi and Escherichia coli were monitored by liquid chromatography-tandem mass spectrometry to test whether a quantitative definition of quorum sensing could help decipher the information content of these signals. Although V. harveyi was able to keep the autoinducer-2 to cell number ratio constant, the ratio of signal to cell number for V. harveyi autoinducer-1 and E. coli autoinducer-2 varied as the cultures grew. These data indicate that V. harveyi uses autoinducer-2 for quorum sensing, while the other molecules may be used to transmit different information or are influenced by metabolic noise.
通过液相色谱-串联质谱监测哈维弧菌和大肠杆菌培养物中的细胞外自动诱导物浓度，以测试定量定义群体感应是否有助于破译这些信号的信息含量。尽管哈维弧菌能够使自动诱导物-2与细胞数量的比例保持不变，但随着培养物的生长，哈维弧菌自动诱导物-1和大肠杆菌自动诱导物-2的信号与细胞数量的比值发生了变化。这些数据表明，哈维弧菌使用自动诱导物-2进行群体感应，而其他分子可能用于传递不同的信息，或者受到代谢噪声的影响。
