Comparative and Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA.
Zebrafish. 2010 Jun;7(2):149-54. doi: 10.1089/zeb.2010.0660.
Visual data collection is paramount for the majority of scientific research. The added transparency of the zebrafish (Danio rerio) allows for a greater detail of complex biological research that accompanies seemingly simple observational tools. We developed a visual data analysis and collection approach that takes advantage of the cylindrical nature of the zebrafish allowing for an efficient and effective method for image capture that we call Specimen in a Corrected Optical Rotational Enclosure imaging. To achieve a nondistorted image, zebrafish were placed in a fluorinated ethylene propylene tube with a surrounding optically corrected imaging solution (water). By similarly matching the refractive index of the housing (fluorinated ethylene propylene tubing) to that of the inner liquid and outer liquid (water), distortion was markedly reduced, producing a crisp imagable specimen that is able to be fully rotated 360 degrees. A similar procedure was established for fixed zebrafish embryos using convenient, readily available borosilicate capillaries surrounded by 75% glycerol. The method described here could be applied to chemical genetic screening and other related high-throughput methods within the fish community and among other scientific fields.
对大多数科学研究来说，视觉数据的收集至关重要。斑马鱼（Danio rerio）的高透明度使得能够更详细地研究复杂的生物学，同时还可以配合看似简单的观察工具。我们开发了一种视觉数据分析和收集方法，利用了斑马鱼的圆柱形状，为图像采集提供了一种高效、有效的方法，我们称之为在校正光学旋转外壳中的标本成像。为了获得无失真的图像，将斑马鱼放置在一个含氟乙烯丙烯管中，周围是经过光学校正的成像溶液（水）。通过使外壳（氟乙烯丙烯管）的折射率与内部液体和外部液体（水）的折射率相匹配，可以显著减少失真，从而产生清晰的可成像标本，可以完全旋转 360 度。对于固定的斑马鱼胚胎，我们采用了类似的方法，使用方便、易于获得的硼硅酸盐毛细管，周围是 75%的甘油。这里描述的方法可以应用于鱼类群体中的化学遗传筛选和其他相关高通量方法，以及其他科学领域。
