Hoeppner Luke H
The Hormel Institute, University of Minnesota, Austin, MN, USA.
Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
Methods Mol Biol. 2022;2475:339-350. doi: 10.1007/978-1-0716-2217-9_25.
Vascular endothelial growth factor (VEGF) stimulates vascular permeability in a variety of human pathologies, such as cancer, ischemic stroke, cardiovascular disease, retinal conditions, and COVID-19-associated pulmonary edema, sepsis, acute lung injury, and acute respiratory distress syndrome. Comprehensive investigation of the molecular mechanisms of VEGF-induced vascular permeability has been hindered by the lack of in vivo models that easily facilitate genetic manipulation studies in real time. To address this need, we generated a heat-inducible VEGF transgenic zebrafish model of vascular permeability. Here, we describe how this zebrafish model can be used to monitor VEGF-induced vascular permeability through live in vivo imaging to identify genetic regulators that play key roles in vascular barrier integrity in physiological conditions and human disease processes.
血管内皮生长因子（VEGF）在多种人类疾病中刺激血管通透性，如癌症、缺血性中风、心血管疾病、视网膜疾病以及与COVID-19相关的肺水肿、脓毒症、急性肺损伤和急性呼吸窘迫综合征。由于缺乏易于实时促进基因操作研究的体内模型，对VEGF诱导血管通透性的分子机制的全面研究受到了阻碍。为满足这一需求，我们构建了一种热诱导的VEGF转基因斑马鱼血管通透性模型。在此，我们描述了如何利用这种斑马鱼模型通过活体体内成像监测VEGF诱导的血管通透性，以识别在生理条件和人类疾病过程中对血管屏障完整性起关键作用的基因调节因子。
