Division of Biotechnology & Bioengineering, Institute of Bioscience and Biotechnology, Kangwon National University, Chunchon, Korea.
Drug Dev Ind Pharm. 2011 Jan;37(1):56-61. doi: 10.3109/03639045.2010.491830. Epub 2010 Jun 9.
Monoolein (MO) cubic phase containing acidic proteinoid was prepared for a pH-dependent release.
The acidic proteinoid was prepared by a thermal-condensation reaction of Asp and Leu (9.85/0.15 in molar ratio). To prepare MO cubic phase, molten MO was hydrated with the proteinoid solution in distilled water. For pH-dependent release experiment, amaranth was included as an anionic dye, and either auramine O or methylene blue was contained as a cationic dye.
The release of amaranth from the cubic phase was promoted under neutral and alkali conditions, possibly because of electrostatic repulsions between the anionic dye and the ionized carboxylic group of the acidic proteinoid. On the contrary, the releases of auramine O and methylene blue were suppressed under neutral and alkali conditions, probably because of electrostatic interactions between the cationic dyes and the ionized carboxylic group.
The acidic proteinoid is believed to control the releases in response to change in pH.
制备含有酸性蛋白的单油酸甘油酯立方相，以实现 pH 依赖性释放。
酸性蛋白通过 Asp 和 Leu（摩尔比为 9.85/0.15）的热缩合反应制备。为了制备 MO 立方相，将熔融 MO 与蛋白溶液在蒸馏水中水合。对于 pH 依赖性释放实验，苋菜被用作阴离子染料，吖啶橙或亚甲蓝被用作阳离子染料。
在中性和碱性条件下，苋菜从立方相中的释放得到促进，这可能是由于阴离子染料与酸性蛋白的离子化羧酸基团之间的静电排斥作用。相反，吖啶橙和亚甲蓝的释放则受到抑制，这可能是由于阳离子染料与离子化羧酸基团之间的静电相互作用。
酸性蛋白被认为可以根据 pH 的变化控制释放。
