Department of Radiobiology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, India.
J Environ Pathol Toxicol Oncol. 2010;29(1):69-79. doi: 10.1615/jenvironpatholtoxicoloncol.v29.i1.90.
The present study was undertaken to investigate the radiation mitigating effect of hydro-alcoholic extract of Saraca indica (SIE) against mice exposed to whole body gamma radiation. Free radical scavenging ability by SIE was demonstrated using hydroxyl, superoxide anion, ABTS*+ and DPPH radicals generated in vitro. A significant increase in the animal survival (dose reduction factor = 1.39), inhibition in the decline of hematological parameters as well as increased number of spleen colony-forming units was observed when SIE was administered prior to radiation. SIE pretreatment increased the levels of glutathione, glutathione S- trans-ferase, catalase and lowered lipid peroxidation. Our findings for the first time demonstrate the protective potential of SIE against radiation induced syndromes with an optimal dose of 400 mg/kg b.wt. The radiation mitigating effect may be attributed to the mechanisms such as free radical scavenging, elevation in antioxidant status, and reduction in lipid peroxidation.
本研究旨在探讨水醇提取物 Saraca indica（SIE）对全身γ 射线照射小鼠的辐射缓解作用。通过在体外生成羟基、超氧阴离子、ABTS*+ 和 DPPH 自由基，证明了 SIE 的自由基清除能力。当 SIE 在辐射前给予时，观察到动物存活率显著增加（剂量减少因子=1.39），血液学参数下降得到抑制，脾集落形成单位数量增加。SIE 预处理增加了谷胱甘肽、谷胱甘肽 S-转移酶、过氧化氢酶的水平，并降低了脂质过氧化。我们的研究结果首次证明了 SIE 对辐射诱导综合征的保护潜力，最佳剂量为 400mg/kg 体重。辐射缓解作用可能归因于自由基清除、抗氧化状态升高和脂质过氧化减少等机制。
