Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Acta Ophthalmol. 2011 Mar;89(2):e115-21. doi: 10.1111/j.1755-3768.2010.01916.x.
Fibrotic disorders are associated with activation of fibroblasts into extracellular matrix-secreting myofibroblasts expressing α-smooth muscle actin (α-SMA). Myofibroblasts are the predominant cellular component of proliferative vitreoretinopathy (PVR) epiretinal membranes. We investigated the expression of molecules involved in myofibroblast activation, migration and proliferation in PVR epiretinal membranes.
Fifteen membranes were studied by immunohistochemical techniques using monoclonal and polyclonal antibodies directed against snail, fibroblast activation protein (FAP), CD44, hydrogen peroxide-inducible clone-5 (Hic-5), galectin-3, interleukin-13 receptor α2 (IL-13Rα2) and receptor for advanced glycation end products (RAGE).
Myofibroblasts expressing α-SMA were present in all membranes. Myofibroblasts expressed nuclear immunoreactivity for Snail and Hic-5, cytoplasmic immunoreactivity for FAP, IL-13Rα2 and RAGE and membranous immunoreactivity for CD44. There was no immunoreactivity for galectin-3. The number of cells expressing α-SMA correlated significantly with the number of cells expressing Snail (r = 0.56; p = 0.03), Hic-5 (r = 0.526; p = 0.044), IL-13Rα2 (r = 0.773; p = 0.001) and RAGE (r = 0.734; p = 0.002).
Snail, FAP, CD44, Hic-5, IL13Rα2 and RAGE may be involved in proliferative events occurring in PVR.
纤维化疾病与成纤维细胞激活为表达α-平滑肌肌动蛋白（α-SMA）的细胞外基质分泌型肌成纤维细胞有关。肌成纤维细胞是增殖性玻璃体视网膜病变（PVR）视网膜前膜的主要细胞成分。我们研究了 PVR 视网膜前膜中参与肌成纤维细胞激活、迁移和增殖的分子表达。
使用针对蜗牛、成纤维细胞激活蛋白（FAP）、CD44、过氧化氢诱导克隆-5（Hic-5）、半乳糖凝集素-3、白细胞介素-13 受体α2（IL-13Rα2）和晚期糖基化终产物受体（RAGE）的单克隆和多克隆抗体，通过免疫组织化学技术研究了 15 个膜。
所有膜中均存在表达α-SMA 的肌成纤维细胞。肌成纤维细胞表达蜗牛和 Hic-5 的核免疫反应性、FAP、IL-13Rα2 和 RAGE 的细胞质免疫反应性以及 CD44 的膜免疫反应性。没有半乳糖凝集素-3 的免疫反应性。表达α-SMA 的细胞数量与表达蜗牛（r = 0.56；p = 0.03）、Hic-5（r = 0.526；p = 0.044）、IL-13Rα2（r = 0.773；p = 0.001）和 RAGE（r = 0.734；p = 0.002）的细胞数量呈显著相关。
蜗牛、FAP、CD44、Hic-5、IL13Rα2 和 RAGE 可能参与 PVR 中发生的增殖事件。
