Botucatu Medical School, UNESP - Univ Estadual Paulista, Department of Gynecology and Obstetrics, Laboratory of Experimental Research in Gynecology and Obstetrics, São Paulo State, Brazil.
Diabetol Metab Syndr. 2010 Jun 8;2(1):37. doi: 10.1186/1758-5996-2-37.
Many experimental studies have been performed to evaluate mild diabetes effects. However, results are divergent regarding glycemia and insulin measurement, fetal macrossomia, and placental weights. The aim was to investigate repercussions of neonatally-induced mild diabetes on the maternal organism and presence of congenital defects in their offspring in other mild diabetes model. On the day of birth, female offspring were distributed into two groups: Group streptozotocin (STZ): received 100 mg STZ/kg body weight, and Control Group: received vehicle in a similar time period. Maternal weights and glycemias were determined at days 0, 7, 14 and 21 of pregnancy. At day 21 of pregnancy, the rats were anesthetized and a laparotomy was performed to weigh and analyze living fetuses and placentas. The fetuses were classified as small (SPA), appropriate (APA) and large (LPA) for pregnancy age. Fetuses were also analyzed for the presence of external anomalies and processed for skeletal anomaly and ossification sites analysis. Statistical significance was considered as p < 0.05. In STZ group, there was increased glycemia at 0 and 14 days of pregnancy, lower weights throughout pregnancy, higher placental weight and index, an increased proportion of fetuses classified as SPA and LPA, and their fetuses presented with an increased frequency of abnormal sternebra, and absent cervical nuclei, which were not enough to cause the emergence of skeletal anomalies. Thus, this study shows that mild diabetes altered fetal development, characterized by intrauterine growth restriction. Further, the reached glycemia does not lead to any major congenital defects in the fetuses of streptozotocin-induced mild diabetic rats.
许多实验研究已经进行，以评估轻度糖尿病的影响。然而，关于血糖和胰岛素测量、胎儿巨大儿和胎盘重量的结果存在分歧。目的是研究新生儿诱导的轻度糖尿病对母体器官的影响，以及在另一种轻度糖尿病模型中其后代是否存在先天性缺陷。在出生当天，雌性后代被分为两组：链脲佐菌素（STZ）组：接受 100mg/kg 体重的 STZ，对照组：在相似时间内接受载体。在妊娠第 0、7、14 和 21 天测量母体体重和血糖。在妊娠第 21 天，大鼠被麻醉，进行剖腹手术以称重和分析活胎和胎盘。胎儿根据妊娠年龄分为小（SPA）、适当（APA）和大（LPA）。还分析了胎儿是否存在外部畸形，并对骨骼畸形和骨化部位进行了分析。统计学意义的临界值为 p<0.05。在 STZ 组中，妊娠 0 天和 14 天血糖升高，整个妊娠期间体重降低，胎盘重量和指数增加，SPA 和 LPA 分类的胎儿比例增加，其胎儿胸骨异常的发生率增加，且颈核缺失，这些都不足以导致骨骼畸形的出现。因此，本研究表明，轻度糖尿病改变了胎儿的发育，表现为宫内生长受限。此外，达到的血糖水平不会导致链脲佐菌素诱导的轻度糖尿病大鼠胎儿出现任何重大先天性缺陷。
