Sinzato Yuri Karen, Volpato Gustavo Tadeu, Iessi Isabela Lovizutto, Bueno Aline, Calderon Iracema de Mattos Paranhos, Rudge Marilza Vieira Cunha, Damasceno Débora Cristina
Laboratory of Experimental Research in Gynecology and Obstetrics, Department of Gynecology and Obstetrics, Botucatu Medical School, Universidade Estadual Paulista, Distrito Rubião Júnior 18618000 s/n, Botucatu, SP, Brazil.
Exp Diabetes Res. 2012;2012:108163. doi: 10.1155/2012/108163. Epub 2012 Jun 20.
The aim of this study was to assess placental changes and reproductive outcomes in neonatally induced mild diabetic dams and fetal development in their offspring. At birth, female rats were assigned either to control or diabetic group (100 mg of streptozotocin/Kg, subcutaneously). At adulthood, the female rats were mated. During pregnancy, the blood glucose levels and glucose and insulin tolerance tests were performed. At term, maternal reproductive outcomes, fetal and placental weight, and placental morphology were analyzed. Diabetic rats had smaller number of living fetuses, implantations and corpora lutea, and increased rate of embryonic loss. Placenta showed morphometric alterations in decidua area. Our results showed that mild diabetes was sufficient to trigger alterations in maternal organism leading to impaired decidua development contributing to failure in embryonic implantation and early embryonic losses. Regardless placental decidua alteration, the labyrinth, which is responsible for the maternal-fetal exchanges, showed no morphometric changes contributing to an appropriate fetal development, which was able to maintain normal fetal weight at term in mild diabetic rats. Thus, this experimental model of diabetes induction at the day of birth was more effective to reproduce the reproductive alterations of diabetic women.
本研究的目的是评估新生期诱导的轻度糖尿病母鼠的胎盘变化和生殖结局，以及其后代的胎儿发育情况。出生时，将雌性大鼠分为对照组或糖尿病组（100 mg链脲佐菌素/千克，皮下注射）。成年后，让雌性大鼠交配。在怀孕期间，进行血糖水平以及葡萄糖和胰岛素耐量试验。足月时，分析母体生殖结局、胎儿和胎盘重量以及胎盘形态。糖尿病大鼠存活胎儿、着床和黄体数量较少，胚胎丢失率增加。胎盘在蜕膜面积上显示出形态计量学改变。我们的结果表明，轻度糖尿病足以引发母体机体的改变，导致蜕膜发育受损，进而导致胚胎着床失败和早期胚胎丢失。尽管胎盘蜕膜发生改变，但负责母胎交换的迷路未显示出形态计量学变化，这有助于胎儿正常发育，使得轻度糖尿病大鼠足月时能够维持正常胎儿体重。因此，这种出生当天诱导糖尿病的实验模型在再现糖尿病女性的生殖改变方面更有效。
