Rota S, Fidan I, Muderris T, Yesilyurt E, Lale Z
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
J Laryngol Otol. 2010 Sep;124(9):990-4. doi: 10.1017/S0022215110001416. Epub 2010 Jun 8.
Some researchers have suggested that Epstein-Barr virus may play a role in the pathogenesis of laryngeal malignancies. In order to clarify the role of cytokines in this disease context, the current study aimed to determine the serum levels of cytokines in Epstein-Barr virus DNA positive patients with laryngeal carcinoma.
The study included 10 patients with diagnosed laryngeal carcinoma and Epstein-Barr virus DNA positive tumour tissue samples. The control group comprised 10 Epstein-Barr virus DNA negative patients diagnosed with laryngeal carcinoma, 10 patients with acute Epstein-Barr virus infection and 10 healthy individuals.
Serum cytokine levels were determined by enzyme-linked immunosorbent assay.
The Epstein-Barr virus DNA positive and negative laryngeal carcinoma patients showed no differences regarding serum levels of the following cytokines: interleukins 1beta, 2, 6 and 12, tumour necrosis factor alpha, and interferon gamma. However, serum levels of interleukin 10 and transforming growth factor beta1 were significantly higher in Epstein-Barr virus DNA positive laryngeal carcinoma patients compared with Epstein-Barr virus DNA negative laryngeal carcinoma patients (p < 0.05).
Our results suggest that the cytokines interleukin 10 and transforming growth factor beta1 may act as growth factors in Epstein-Barr virus related laryngeal carcinoma. These cytokines may thus represent potential targets for molecular therapeutic treatment for laryngeal carcinoma; they may also be useful as indicators of disease prognosis.
一些研究人员认为，爱泼斯坦-巴尔病毒可能在喉恶性肿瘤的发病机制中起作用。为了阐明细胞因子在这种疾病背景下的作用，本研究旨在测定爱泼斯坦-巴尔病毒DNA阳性的喉癌患者血清中的细胞因子水平。
该研究纳入了10例经诊断患有喉癌且肿瘤组织样本爱泼斯坦-巴尔病毒DNA呈阳性的患者。对照组包括10例诊断为喉癌但爱泼斯坦-巴尔病毒DNA呈阴性的患者、10例急性爱泼斯坦-巴尔病毒感染患者和10名健康个体。
采用酶联免疫吸附测定法测定血清细胞因子水平。
爱泼斯坦-巴尔病毒DNA阳性和阴性的喉癌患者在以下细胞因子的血清水平方面无差异：白细胞介素1β、2、6和12、肿瘤坏死因子α以及干扰素γ。然而，与爱泼斯坦-巴尔病毒DNA阴性的喉癌患者相比，爱泼斯坦-巴尔病毒DNA阳性的喉癌患者血清白细胞介素10和转化生长因子β1水平显著更高（p < 0.05）。
我们的结果表明，细胞因子白细胞介素10和转化生长因子β1可能在爱泼斯坦-巴尔病毒相关的喉癌中作为生长因子起作用。因此，这些细胞因子可能代表喉癌分子治疗的潜在靶点；它们也可能作为疾病预后的指标。
