Graduate School of Engineering, Tohoku University, Aoba, Sendai, Japan.
Bioorg Med Chem Lett. 2010 Jun 15;20(12):3588-91. doi: 10.1016/j.bmcl.2010.04.122. Epub 2010 May 22.
Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-beta-XXXG and DMT-beta-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-beta-1,4-D-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.
两种 4,6-二甲氧基-1,3,5-三嗪（DMT）寡木糖葡萄糖醛酸苷，DMT-β-XXXG 和 DMT-β-XLLG，已经通过从相应的未保护寡木糖葡萄糖醛酸苷一步法在水中合成。结果表明，DMT 衍生物可被里氏木霉的内切-β-1,4-D-葡聚糖酶 III（EGIII）水解，并可作为测定 EGIII 动力学参数的底物。由于 DMT-糖苷的合成过程非常简单，无需使用保护基团，因此本 DMT 方法将成为研究糖苷水解酶作用的一种方便的分析工具。
