Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-514 Aoba, Sendai, Miyagi 980-8579, Japan.
Org Biomol Chem. 2010 Nov 21;8(22):5126-32. doi: 10.1039/c0ob00190b. Epub 2010 Sep 9.
Novel glycosidic compounds, 4,6-dialkoxy-1,3,5-triazin-2-yl β-lactosides (DAT-β-Lac), have been prepared directly in water from lactose. The reaction was carried out on a laboratory scale without protecting the hydroxy groups of lactose. The resulting triazine derivatives were found to be recognized by endo-β1,4-glucanase III from Trichoderma reesei (EGIII). The EGIII-catalysed transglycosylation of 4,6-dimethoxy-1,3,5-triazine derivative (DMT-β-Lac) with various glycosyl acceptors has successfully been demonstrated, affording the corresponding lactosylated products.
新型糖苷类化合物，4,6-二烷氧基-1,3,5-三嗪-2-基β-乳糖苷（DAT-β-Lac），可直接从乳糖在水中制备。反应在实验室规模下进行，无需保护乳糖的羟基。所得到的三嗪衍生物被发现可被里氏木霉的内切-β1,4-葡聚糖酶 III（EGIII）识别。EGIII 催化的 4,6-二甲氧基-1,3,5-三嗪衍生物（DMT-β-Lac）与各种糖基受体的转糖苷反应已成功进行，得到相应的乳酰化产物。
