Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Anticancer Res. 2010 Apr;30(4):1057-64.
The purpose of our study was to evaluate the application of thermoreversible gelation polymer (TGP) as a local drug delivery system for malignant glioma.
Polymeric microspheres or liposomes loaded with doxorubicin (sphere-dox or lipo-dox) were combined with TGP to provide continuous drug delivery of doxorubicin (dox) for kinetic release studies and cell viability assays on glioma cell lines in vitro. For in vivo studies, TGP loaded with dox alone (TGP-dox) was combined with sphere-dox or lipo-dox. Their antitumor effects on subcutaneous human glioma xenografts were evaluated in nude mice.
In vitro, TGP combined with sphere-dox or lipo-dox released dox for up to 30 days. In vivo, TGP-dox combined with sphere-dox or lipo-dox inhibited subcutaneous glioma tumor growth until day 32 and day 38, respectively.
TGP in combination with microspheres or liposomes successfully prolonged the release of dox and its antitumor effects.
本研究旨在评估温敏凝胶聚合物（TGP）作为恶性脑胶质瘤局部药物递送系统的应用。
载多柔比星的聚合物微球或脂质体（载多柔比星微球或载多柔比星脂质体）与 TGP 联合使用，为动力学释放研究和体外胶质瘤细胞系的细胞活力测定提供多柔比星（多柔比星）的持续药物递送。对于体内研究，单独负载多柔比星的 TGP（TGP-多柔比星）与载多柔比星微球或载多柔比星脂质体联合使用。在裸鼠中评估它们对皮下人胶质母细胞瘤异种移植物的抗肿瘤作用。
体外，TGP 与载多柔比星微球或载多柔比星脂质体联合使用可使多柔比星释放长达 30 天。在体内，TGP-多柔比星与载多柔比星微球或载多柔比星脂质体联合使用分别抑制皮下胶质瘤肿瘤生长至第 32 天和第 38 天。
TGP 与微球或脂质体联合成功延长了多柔比星的释放及其抗肿瘤作用。
