Endocrinology and Diabetes Unit, Hospital Universitari Joan XXIII, C/Dr. Mallafré Guasch 4, Tarragona, Spain.
Am J Physiol Endocrinol Metab. 2010 Aug;299(2):E308-17. doi: 10.1152/ajpendo.00699.2009. Epub 2010 Jun 8.
LPIN1 is a gene with important effects on lipidic and metabolic homeostasis. Human subcutaneous LPIN1 expression levels in adipose tissue are related with a better metabolic profile, including insulin sensitivity markers. However, there are few data on the regulation of LPIN1 in visceral adipose tissue (VAT). Our aim was to perform a cross-sectional analysis of VAT compared with subcutaneous (SAT) LPIN1 expression in a well-characterized obese cohort, its relation with the expression of genes involved in lipid metabolism, and the in vitro response to lipogenic and lipolytic stimuli. A downregulation of total LPIN1 mRNA expression in subjects with obesity was found in VAT similarly to that in SAT. Despite similar total LPIN1 mRNA levels in SAT and VAT, a close relationship with clinical parameters and with many lipogenic and lipolytic genes was observed primarily in SAT depot. As shown in the in vitro analysis, the low-grade proinflammatory environment and the insulin resistance associated with obesity may contribute to downregulate LPIN1 in adipose tissue, leading to a worse metabolic profile.
LPIN1 是一个对脂质和代谢稳态有重要影响的基因。人体脂肪组织中皮下 LPIN1 的表达水平与更好的代谢特征有关，包括胰岛素敏感性标志物。然而，关于内脏脂肪组织 (VAT) 中 LPIN1 的调节的数据很少。我们的目的是在一个特征明确的肥胖队列中对 VAT 与皮下 (SAT) LPIN1 表达进行横断面分析，研究其与参与脂质代谢的基因表达的关系，以及对脂肪生成和脂肪分解刺激的体外反应。我们发现肥胖患者的 VAT 中总 LPIN1 mRNA 表达下调，与 SAT 中的情况相似。尽管 SAT 和 VAT 中的总 LPIN1 mRNA 水平相似，但主要在 SAT 库中观察到与临床参数以及许多脂肪生成和脂肪分解基因的密切关系。如体外分析所示，与肥胖相关的低度炎症环境和胰岛素抵抗可能导致脂肪组织中 LPIN1 的下调，从而导致代谢特征恶化。
