Suppr超能文献

HIV-1 gag和pol基因产物在稳定转染的啮齿动物细胞系中的表达、移码及极低效率的蛋白水解加工。

Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines.

作者信息

Moosmayer D, Reil H, Ausmeier M, Scharf J G, Hauser H, Jentsch K D, Hunsmann G

机构信息

Deutsches Primatenzentrum, Abt. Virologie und Immunologie, Göttingen.

出版信息

Virology. 1991 Jul;183(1):215-24. doi: 10.1016/0042-6822(91)90134-w.

DOI:10.1016/0042-6822(91)90134-w
PMID:2053281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7131782/
Abstract

Expression, ribosomal frameshifting, and proteolytic processing of HIV-1 GAG and POL proteins were investigated in heterologous mammalian cells in order to elucidate the influence of the cellular background on these events. DNA fragments encoded by the gag and pol region were expressed in two rodent cell lines, LTK- and BHK. Both stably transfected cell lines continuously produce recombinant proteins which react with HIV-specific antisera. The GAG precursor and a 39-kDa proteolytic fragment thereof were the major recombinant proteins detected. Expression of the gag-pol region leads to the production of the GAG-POL precursor. Ribosomal frameshifting at the HIV-1 shifty sequence to a typical extent could be positively demonstrated by an enzyme assay. Despite the presence of the viral protease within the GAG-POL precursors, proteolytic processing of the HIV-derived polyproteins was extremely inefficient. The efficiency could not be enhanced by overexpression of the HIV-1 protease encoding region.

摘要

为了阐明细胞背景对这些事件的影响，在异源哺乳动物细胞中研究了HIV-1 GAG和POL蛋白的表达、核糖体移码和蛋白水解加工。由gag和pol区域编码的DNA片段在两种啮齿动物细胞系LTK-和BHK中表达。两种稳定转染的细胞系都持续产生与HIV特异性抗血清反应的重组蛋白。GAG前体及其39 kDa蛋白水解片段是检测到的主要重组蛋白。gag-pol区域的表达导致GAG-POL前体的产生。通过酶测定可以明确证明HIV-1移码序列处的核糖体移码达到典型程度。尽管GAG-POL前体中存在病毒蛋白酶，但HIV衍生多蛋白的蛋白水解加工效率极低。通过过表达HIV-1蛋白酶编码区域并不能提高效率。

相似文献

1
Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines.HIV-1 gag和pol基因产物在稳定转染的啮齿动物细胞系中的表达、移码及极低效率的蛋白水解加工。
Virology. 1991 Jul;183(1):215-24. doi: 10.1016/0042-6822(91)90134-w.
2
Overexpression of the gag-pol precursor from human immunodeficiency virus type 1 proviral genomes results in efficient proteolytic processing in the absence of virion production.来自1型人类免疫缺陷病毒前病毒基因组的gag-pol前体的过表达在不产生病毒粒子的情况下导致有效的蛋白水解加工。
J Virol. 1991 Sep;65(9):5111-7. doi: 10.1128/JVI.65.9.5111-5117.1991.
3
Extensive regions of pol are required for efficient human immunodeficiency virus polyprotein processing and particle maturation.有效的人类免疫缺陷病毒多聚蛋白加工和颗粒成熟需要pol的广泛区域。
Virology. 1996 May 1;219(1):29-36. doi: 10.1006/viro.1996.0219.
4
Natural variation in HIV-1 protease, Gag p7 and p6, and protease cleavage sites within gag/pol polyproteins: amino acid substitutions in the absence of protease inhibitors in mothers and children infected by human immunodeficiency virus type 1.人类免疫缺陷病毒1型感染的母婴中，HIV-1蛋白酶、Gag p7和p6以及gag/pol多蛋白内蛋白酶切割位点的自然变异：无蛋白酶抑制剂情况下的氨基酸替代
Virology. 1996 May 15;219(2):407-16. doi: 10.1006/viro.1996.0266.
5
Mutations of the human immunodeficiency virus type 1 p6Gag domain result in reduced retention of Pol proteins during virus assembly.人类免疫缺陷病毒1型p6Gag结构域的突变导致病毒组装过程中Pol蛋白的保留减少。
J Virol. 1998 Apr;72(4):3412-7. doi: 10.1128/JVI.72.4.3412-3417.1998.
6
Analysis of protein expression and virus-like particle formation in mammalian cell lines stably expressing HIV-1 gag and env gene products with or without active HIV proteinase.在稳定表达HIV-1 gag和env基因产物且有或无活性HIV蛋白酶的哺乳动物细胞系中分析蛋白质表达和病毒样颗粒形成。
Virology. 1993 Feb;192(2):605-17. doi: 10.1006/viro.1993.1077.
7
Extended nucleocapsid protein is cleaved from the Gag-Pol precursor of human immunodeficiency virus type 1.延伸核衣壳蛋白是从1型人类免疫缺陷病毒的Gag-Pol前体中切割而来的。
J Gen Virol. 2001 Mar;82(Pt 3):581-590. doi: 10.1099/0022-1317-82-3-581.
8
A bipartite membrane-binding signal in the human immunodeficiency virus type 1 matrix protein is required for the proteolytic processing of Gag precursors in a cell type-dependent manner.人类免疫缺陷病毒1型基质蛋白中的一种二分体膜结合信号以细胞类型依赖的方式参与Gag前体的蛋白水解加工。
J Virol. 1998 Nov;72(11):9061-8. doi: 10.1128/JVI.72.11.9061-9068.1998.
9
Morphogenesis of recombinant HIV-2 gag core particles.
Virus Res. 1992 Jul;24(2):197-210. doi: 10.1016/0168-1702(92)90007-v.
10
Proteolytic activity of novel human immunodeficiency virus type 1 proteinase proteins from a precursor with a blocking mutation at the N terminus of the PR domain.来自一种在蛋白酶（PR）结构域N端带有阻断突变的前体的新型人类免疫缺陷病毒1型蛋白酶蛋白的蛋白水解活性。
J Virol. 1994 Jan;68(1):240-50. doi: 10.1128/JVI.68.1.240-250.1994.

引用本文的文献

1
Specific effects of ribosome-tethered molecular chaperones on programmed -1 ribosomal frameshifting.核糖体连接分子伴侣对程序性-1核糖体移码的特异性影响。
Eukaryot Cell. 2006 Apr;5(4):762-70. doi: 10.1128/EC.5.4.762-770.2006.
2
Chimeric human immunodeficiency virus type 1 containing murine leukemia virus matrix assembles in murine cells.包含鼠白血病病毒基质的嵌合型1型人类免疫缺陷病毒在鼠细胞中组装。
J Virol. 2002 Jan;76(1):436-43. doi: 10.1128/jvi.76.1.436-443.2002.
3
A block to human immunodeficiency virus type 1 assembly in murine cells.小鼠细胞中1型人类免疫缺陷病毒组装的一个障碍。
J Virol. 2000 Apr;74(8):3859-70. doi: 10.1128/jvi.74.8.3859-3870.2000.
4
Astrovirus ribosomal frameshifting in an infection-transfection transient expression system.星状病毒在感染-转染瞬时表达系统中的核糖体移码
J Virol. 1996 May;70(5):2869-75. doi: 10.1128/JVI.70.5.2869-2875.1996.
5
Translational frameshifting mediated by a viral sequence in plant cells.植物细胞中由病毒序列介导的翻译移码
Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2262-6. doi: 10.1073/pnas.89.6.2262.
6
Human immunodeficiency virus type 1 gag-pol frameshifting is dependent on downstream mRNA secondary structure: demonstration by expression in vivo.1型人类免疫缺陷病毒gag-pol移码依赖于下游mRNA二级结构：体内表达的证明
J Virol. 1992 Aug;66(8):5147-51. doi: 10.1128/JVI.66.8.5147-5151.1992.

本文引用的文献

1
DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.从对溴脱氧尿苷有抗性的L细胞中删除胸苷激酶活性。
Exp Cell Res. 1963 Aug;31:297-312. doi: 10.1016/0014-4827(63)90007-7.
2
A new dominant hybrid selective marker for higher eukaryotic cells.一种用于高等真核细胞的新型显性杂交选择标记。
J Mol Biol. 1981 Jul 25;150(1):1-14. doi: 10.1016/0022-2836(81)90321-1.
3
A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
Virology. 1973 Apr;52(2):456-67. doi: 10.1016/0042-6822(73)90341-3.
4
Firefly luciferase gene: structure and expression in mammalian cells.萤火虫荧光素酶基因：在哺乳动物细胞中的结构与表达
Mol Cell Biol. 1987 Feb;7(2):725-37. doi: 10.1128/mcb.7.2.725-737.1987.
5
Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.来自白色链霉菌的赋予嘌呤霉素抗性的基因在哺乳动物细胞中的表达。
Nucleic Acids Res. 1986 Jun 11;14(11):4617-24. doi: 10.1093/nar/14.11.4617.
6
HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.人类嗜T淋巴细胞病毒III型（HTLV-III）的表达和产生涉及病毒RNA剪接和翻译水平的复杂调控。
Cell. 1986 Sep 12;46(6):807-17. doi: 10.1016/0092-8674(86)90062-0.
7
Expression of the human immunodeficiency virus gag gene in insect cells.
Virology. 1987 May;158(1):248-50. doi: 10.1016/0042-6822(87)90262-5.
8
An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV.冠状病毒传染性支气管炎病毒（IBV）聚合酶编码区中的一种高效核糖体移码信号。
EMBO J. 1987 Dec 1;6(12):3779-85. doi: 10.1002/j.1460-2075.1987.tb02713.x.
9
Immunodeficiency virus rev trans-activator modulates the expression of the viral regulatory genes.免疫缺陷病毒反式转录激活因子调节病毒调控基因的表达。
Nature. 1988 Sep 8;335(6186):181-3. doi: 10.1038/335181a0.
10
Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor.在大肠杆菌中表达的人类免疫缺陷病毒蛋白酶表现出gag前体的自身加工和特异性成熟。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8903-6. doi: 10.1073/pnas.84.24.8903.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验