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Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines.HIV-1 gag和pol基因产物在稳定转染的啮齿动物细胞系中的表达、移码及极低效率的蛋白水解加工。
Virology. 1991 Jul;183(1):215-24. doi: 10.1016/0042-6822(91)90134-w.
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HIV-1 gag和pol基因产物在稳定转染的啮齿动物细胞系中的表达、移码及极低效率的蛋白水解加工。

Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines.

作者信息

Moosmayer D, Reil H, Ausmeier M, Scharf J G, Hauser H, Jentsch K D, Hunsmann G

机构信息

Deutsches Primatenzentrum, Abt. Virologie und Immunologie, Göttingen.

出版信息

Virology. 1991 Jul;183(1):215-24. doi: 10.1016/0042-6822(91)90134-w.

DOI:10.1016/0042-6822(91)90134-w
PMID:2053281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7131782/
Abstract

Expression, ribosomal frameshifting, and proteolytic processing of HIV-1 GAG and POL proteins were investigated in heterologous mammalian cells in order to elucidate the influence of the cellular background on these events. DNA fragments encoded by the gag and pol region were expressed in two rodent cell lines, LTK- and BHK. Both stably transfected cell lines continuously produce recombinant proteins which react with HIV-specific antisera. The GAG precursor and a 39-kDa proteolytic fragment thereof were the major recombinant proteins detected. Expression of the gag-pol region leads to the production of the GAG-POL precursor. Ribosomal frameshifting at the HIV-1 shifty sequence to a typical extent could be positively demonstrated by an enzyme assay. Despite the presence of the viral protease within the GAG-POL precursors, proteolytic processing of the HIV-derived polyproteins was extremely inefficient. The efficiency could not be enhanced by overexpression of the HIV-1 protease encoding region.

摘要

为了阐明细胞背景对这些事件的影响，在异源哺乳动物细胞中研究了HIV-1 GAG和POL蛋白的表达、核糖体移码和蛋白水解加工。由gag和pol区域编码的DNA片段在两种啮齿动物细胞系LTK-和BHK中表达。两种稳定转染的细胞系都持续产生与HIV特异性抗血清反应的重组蛋白。GAG前体及其39 kDa蛋白水解片段是检测到的主要重组蛋白。gag-pol区域的表达导致GAG-POL前体的产生。通过酶测定可以明确证明HIV-1移码序列处的核糖体移码达到典型程度。尽管GAG-POL前体中存在病毒蛋白酶，但HIV衍生多蛋白的蛋白水解加工效率极低。通过过表达HIV-1蛋白酶编码区域并不能提高效率。