来自白色链霉菌的赋予嘌呤霉素抗性的基因在哺乳动物细胞中的表达。
Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.
作者信息
Vara J A, Portela A, Ortín J, Jiménez A
出版信息
Nucleic Acids Res. 1986 Jun 11;14(11):4617-24. doi: 10.1093/nar/14.11.4617.
Abstract
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.
摘要
编码来自白色链霉菌的嘌呤霉素N - 乙酰转移酶的基因已被克隆到哺乳动物细胞 - 大肠杆菌穿梭载体中SV40早期启动子旁边。当将这种构建体导入VERO细胞时，它表达了相关的酶活性。此外，嘌呤霉素N - 乙酰转移酶基因已被用作显性标记，用于选择能够在抗生素存在下生长的转化哺乳动物细胞。
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Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.来自白色链霉菌的赋予嘌呤霉素抗性的基因在哺乳动物细胞中的表达。
Nucleic Acids Res. 1986 Jun 11;14(11):4617-24. doi: 10.1093/nar/14.11.4617.
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