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1
Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.来自白色链霉菌的赋予嘌呤霉素抗性的基因在哺乳动物细胞中的表达。
Nucleic Acids Res. 1986 Jun 11;14(11):4617-24. doi: 10.1093/nar/14.11.4617.
2
Cloning and expression of a puromycin N-acetyl transferase gene from Streptomyces alboniger in Streptomyces lividans and Escherichia coli.来自白色链霉菌的嘌呤霉素N - 乙酰转移酶基因在变铅青链霉菌和大肠杆菌中的克隆与表达。
Gene. 1985;33(2):197-206. doi: 10.1016/0378-1119(85)90094-0.
3
Two genes in Streptomyces alboniger puromycin biosynthesis pathway are closely linked.白色链霉菌嘌呤霉素生物合成途径中的两个基因紧密连锁。
Gene. 1988 Sep 15;69(1):135-40. doi: 10.1016/0378-1119(88)90386-1.
4
Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, puromycin, and its regulated expression in heterologous hosts.氨基核苷类抗生素嘌呤霉素完整生物合成基因簇的克隆及其在异源宿主中的调控表达。
EMBO J. 1992 Feb;11(2):785-92. doi: 10.1002/j.1460-2075.1992.tb05112.x.
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Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger.来自白色链霉菌的编码嘌呤霉素N-乙酰基转移酶的pac基因的分子分析。
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Identification of the gene encoding an N-acetylpuromycin N-acetylhydrolase in the puromycin biosynthetic gene cluster from Streptomyces alboniger.从白色链霉菌嘌呤霉素生物合成基因簇中鉴定编码N-乙酰嘌呤霉素N-乙酰水解酶的基因。
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The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin.来自白色链霉菌嘌呤簇的pur8基因编码一种高度疏水的多肽，该多肽赋予对嘌呤霉素的抗性。
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The mechanism of resistance to puromycin and to the puromycin-precursor O-demethyl-puromycin in Streptomyces alboniger.白色链霉菌对嘌呤霉素及嘌呤霉素前体O-去甲基嘌呤霉素的抗性机制
J Gen Microbiol. 1985 Nov;131(11):2877-83. doi: 10.1099/00221287-131-11-2877.

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Isolation and characterization of puromycin-resistant clones from cultured mosquito cells.从培养的蚊子细胞中分离和鉴定嘌呤霉素抗性克隆。
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
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来自白色链霉菌的赋予嘌呤霉素抗性的基因在哺乳动物细胞中的表达。

Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.

作者信息

Vara J A, Portela A, Ortín J, Jiménez A

出版信息

Nucleic Acids Res. 1986 Jun 11;14(11):4617-24. doi: 10.1093/nar/14.11.4617.

DOI:10.1093/nar/14.11.4617
PMID:3714487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311469/
Abstract

The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.

摘要

编码来自白色链霉菌的嘌呤霉素N - 乙酰转移酶的基因已被克隆到哺乳动物细胞 - 大肠杆菌穿梭载体中SV40早期启动子旁边。当将这种构建体导入VERO细胞时，它表达了相关的酶活性。此外，嘌呤霉素N - 乙酰转移酶基因已被用作显性标记，用于选择能够在抗生素存在下生长的转化哺乳动物细胞。