尿道周围神经肌肉电刺激对大鼠排尿频率的影响。

Effects of periurethral neuromuscular electrical stimulation on the voiding frequency in rats.

作者信息

Zhang Yingchun, Bicek Andrew D, Wang Guangjian, Timm Gerald W

机构信息

Department of Urologic Surgery, University of Minnesota, 748 Mayo Memorial Building, 420 Delaware Street S.E, Minneapolis, MN 55455, USA.

出版信息

Int Urogynecol J. 2010 Oct;21(10):1279-84. doi: 10.1007/s00192-010-1189-y. Epub 2010 Jun 2.

DOI:10.1007/s00192-010-1189-y

PMID:20532871

Abstract

INTRODUCTION AND HYPOTHESIS

This study aims to test the hypothesis that a urethra-to-bladder inhibitory pathway exists through which periurethral neuromuscular electrical stimulation (NMES) inhibits overactive bladder contractions in rats.

METHODS

Bladder overactivity was induced in 22 female Sprague Dawley rats by injection of ketamine/xylazine/acepromizine (K/X/A). A bipolar electrode was placed surgically in the periurethral region to deliver NMES. Intravesical pressure, bladder inter-contraction interval (ICI) and voided volume (VV) were monitored while the bladder was continuously infused with saline.

RESULTS

K/X/A induced more frequent bladder contractions (ICI = 48.6 +/- 20.1 s, before cutting the pubo-symphasis) compared to a 10-min ICI induced by urethane. NMES significantly increased ICI (63.1 +/- 31.3 s before vs. 97.2 +/- 42.9 s after NMES, p < 0.001) and VV (0.063 = 0.041 ml before vs. 0.088 = 0.044 ml after NMES, p < 0.02).

CONCLUSIONS

Injection of K/X/A may potentially be used as a model of bladder overactivity. NMES inhibits bladder contractions in rats with bladder overactivity, which supports the existence of a urethra-to-bladder inhibitory pathway.

摘要

引言与假设

本研究旨在验证以下假设，即存在一条从尿道到膀胱的抑制性通路，通过该通路，尿道周围神经肌肉电刺激（NMES）可抑制大鼠膀胱过度收缩。

方法

通过注射氯胺酮/赛拉嗪/乙酰丙嗪（K/X/A）诱导22只雌性Sprague Dawley大鼠出现膀胱过度活动。手术将双极电极置于尿道周围区域以进行NMES。在膀胱持续灌注生理盐水的同时，监测膀胱内压、膀胱收缩间期（ICI）和排尿量（VV）。

结果

与氨基甲酸乙酯诱导的10分钟ICI相比，K/X/A诱导的膀胱收缩更频繁（在切断耻骨联合前，ICI = 48.6 +/- 20.1秒）。NMES显著增加了ICI（NMES前为63.1 +/- 31.3秒，NMES后为97.2 +/- 42.9秒，p < 0.001）和VV（NMES前为0.063 = 0.041毫升，NMES后为0.088 = 0.044毫升，p < 0.02）。