Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
Anal Bioanal Chem. 2010 Aug;397(7):2997-3003. doi: 10.1007/s00216-010-3805-6. Epub 2010 Jun 9.
A novel enzyme reactor was prepared using calcium alginate fiber (CAF) and amine-modified nanosized mesoporous silica (AMNMS) as a support. Combination of the adsorption of the enzyme on AMNMS with the cage effect of the polymer greatly increases the catalytic activity and the stability of the immobilized enzyme. It was shown that the lifetime, stability, and catalytic activity of the enzyme reactor were greatly improved by incorporating AMNMS into CAF to efficiently encapsulate the enzyme. Glucose oxidase was chosen as a model enzyme to explore the possibility of using CAF-AMNMS as a matrix for enzyme immobilization in the design of a chemiluminescence (CL) flow-through biosensor. The sensitivity of the flow-through biosensor combined with a novel luminol-diperiodatonickelate CL system was higher than for other reported CL biosensors. The proposed biosensor exhibits short response time, easy operation, long lifetime, high catalytic activity, high sensitivity, and simple assembly.
一种新型的酶反应器是使用海藻酸钠纤维（CAF）和胺改性纳米介孔硅（AMNMS）作为载体制备的。将酶吸附在 AMNMS 上与聚合物的笼状效应相结合，极大地提高了固定化酶的催化活性和稳定性。结果表明，通过将 AMNMS 掺入 CAF 中可以有效地包埋酶，从而极大地提高了酶反应器的寿命、稳定性和催化活性。葡萄糖氧化酶被选为模型酶，以探索 CAF-AMNMS 作为酶固定化基质在设计化学发光（CL）流通生物传感器中的可能性。结合新型鲁米诺-过二硫酸盐 CL 系统的流通生物传感器的灵敏度高于其他报道的 CL 生物传感器。所提出的生物传感器具有响应时间短、操作简单、寿命长、催化活性高、灵敏度高、组装简单等特点。
