MacFarlane S A, Shanks M, Davies J W, Zlotnick A, Lomonossoff G P
Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, United Kingdom.
Virology. 1991 Jul;183(1):405-9. doi: 10.1016/0042-6822(91)90155-5.
The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this protein have been identified by comparison with the known three-dimensional structure of the virion and cleavage at these sites would lead to a range of products consistent with those observed during processing of the M RNA-encoded polyproteins in vitro. We have performed computer-aided searches for reiterated sequences within BPMV M RNA which might explain why ordered RNA is visible in the electron density map of BPMV middle component particles (Chen, Z., Stauffacher, C. V., Li, Y., Schmidt, T., Bomu, W., Kamer, G., Shanks, M., Lomonossoff, G., and Johnson, J. E., 1989, Science 245, 154-159). These searches revealed both the presence of overrepresented pentameric sequences and a consensus sequence which was repeated 15 times within the RNA sequence.
菜豆荚斑驳病毒（BPMV）中间组分RNA（M RNA）的完整核苷酸序列已被确定。该序列由3662个核苷酸组成，包含一个单一的长开放阅读框，足以编码一个113353道尔顿的蛋白质。通过与已知病毒粒子的三维结构进行比较，已确定该蛋白质内的蛋白水解加工位点，在这些位点的切割将产生一系列与体外M RNA编码的多蛋白加工过程中观察到的产物一致的产物。我们已对BPMV M RNA中的重复序列进行了计算机辅助搜索，这可能解释了为什么在BPMV中间组分颗粒的电子密度图中可以看到有序的RNA（Chen, Z., Stauffacher, C. V., Li, Y., Schmidt, T., Bomu, W., Kamer, G., Shanks, M., Lomonossoff, G., and Johnson, J. E., 1989, Science 245, 154 - 159）。这些搜索揭示了过度表达的五聚体序列的存在以及一个在RNA序列中重复15次的共有序列。
