Human enteric adenovirus type 41 binding to HEp-2 cells is specific.

Properties of human enteric adenovirus type 41 (Ad41) binding to its receptor on the surface of HEp-2 cells were investigated. The binding was found to be temperature-dependent, saturable, and specific. Analysis of the binding data showed a single class of 4.3 x 10(4) receptor sites per cell, an equilibrium dissociation constant of 21.0 nM, and no cooperativity among receptor sites. Trypsin-treated HEp-2 cells subsequently grown in the presence of tunicamycin or 2-deoxyglucose recovered full Ad41 binding activity, but could not if subsequently grown in the presence of cycloheximide. These data indicate that a single type of virus receptor, likely protein in nature, is present on the surface of HEp-2 cells to specifically bind Ad41.