Persson R, Svensson U, Everitt E
J Virol. 1983 Jun;46(3):956-63. doi: 10.1128/JVI.46.3.956-963.1983.
Adenovirus type 2 attachment to HeLa cells was analyzed under controlled conditions. The temperature-dependent attachment kinetics revealed an inflection point at around 20 degrees C, and above this temperature the increase of the rate was doubled. In multiplicity dependence experiments, the attachment exhibited positive cooperative binding at 37 degrees C. This binding pattern was inhibited by low temperatures and prefixation of cells with 0.015% glutaraldehyde. Attachment of rhodamine-labeled virions revealed capping of the particles on 15% of the cells at 37 degrees C. Capping was inhibited by low temperatures, glutaraldehyde fixation of cells, and treatment with cytochalasin B, azide, and 2-deoxyglucose. Consequently, we propose that the adenovirus type 2 attachment to cells leads to rearrangements in the plasma membrane, resulting in cooperative binding and capping of the virus particles.
在可控条件下分析了2型腺病毒与HeLa细胞的附着情况。温度依赖性附着动力学显示在约20℃处有一个拐点，高于此温度速率增加一倍。在多重依赖性实验中，附着在37℃时表现出正协同结合。这种结合模式受到低温以及用0.015%戊二醛对细胞进行预固定的抑制。罗丹明标记病毒粒子的附着显示在37℃时15%的细胞上有粒子加帽现象。加帽受到低温、细胞的戊二醛固定以及用细胞松弛素B、叠氮化物和2-脱氧葡萄糖处理的抑制。因此，我们提出2型腺病毒与细胞的附着导致质膜重排，从而产生病毒粒子的协同结合和加帽。
