Yamafuji R, Watanabe Y, Meshi T, Okada Y
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Virology. 1991 Jul;183(1):99-105. doi: 10.1016/0042-6822(91)90122-r.
Tm-1 is a gene that provides resistance to tomato plants against tobacco mosaic virus (TMV) infection. In tomato cells carrying the Tm-1 gene, multiplication of TMV is inhibited. From previous analysis of resistance-breaking mutants, the involvement of the 130- and 180-kDa proteins, putative viral replicases, in the resistance conferred by the Tm-1 gene was suggested. When wild-type TMV RNA was co-inoculated with a resistance-breaking mutant RNA, replication of the wild-type TMV genomic RNA could not be rescued by the 130- and 180-kDa proteins of a resistance-breaking strain, Lta1. To investigate how the putative resistance factor interacts with the 130- and 180-kDa proteins, we expressed the wild-type TMV protein sequence that is associated with the resistance-breaking phenomenon as part of a recombinant virus derived from Lta1 in Tm-1/Tm-1 protoplasts. No specific degradation of wild-type TMV protein sequences was observed, suggesting that the mechanism of the resistance does not involve the instability of a viral protein.
Tm-1是一种使番茄植株对烟草花叶病毒（TMV）感染产生抗性的基因。在携带Tm-1基因的番茄细胞中，TMV的增殖受到抑制。根据之前对抗性破坏突变体的分析，推测病毒复制酶130 kDa和180 kDa蛋白参与了Tm-1基因赋予的抗性。当野生型TMV RNA与一个抗性破坏突变体RNA共同接种时，抗性破坏菌株Lta1的130 kDa和180 kDa蛋白无法拯救野生型TMV基因组RNA的复制。为了研究推测的抗性因子如何与130 kDa和180 kDa蛋白相互作用，我们在Tm-1/Tm-1原生质体中，将与抗性破坏现象相关的野生型TMV蛋白序列作为源自Lta1的重组病毒的一部分进行表达。未观察到野生型TMV蛋白序列的特异性降解，这表明抗性机制不涉及病毒蛋白的不稳定性。
