Watanabe Y, Kishibayashi N, Motoyoshi F, Okada Y
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Virology. 1987 Dec;161(2):527-32. doi: 10.1016/0042-6822(87)90147-4.
Tm-1 is a gene which confers resistance to infection, in tomatoes, by tobacco mosaic virus (TMV). To investigate the biochemical mechanism of the resistance, we have established cell suspensions of three lines of tomatoes, i.e., +/+ (susceptible, wild-type, no Tm-1 gene), Tm-1/+ (heterozygous for the Tm-1 gene), and Tm-1/Tm-1 (homozygous for the Tm-1 gene). Protoplasts isolated from these cells were inoculated with RNA of the tomato strain L and Lta1 (a resistance-breaking strain which was recently isolated spontaneously from L) of TMV by means of electrophoration. The syntheses of all viral-coded proteins and TMV-specific RNAs could be detected in L-inoculated +/+ and Lta1-inoculated +/+, Tm-1/+, Tm-1/Tm-1 protoplasts, while their production was markedly reduced in L-inoculated Tm-1/+ protoplasts. L strain could multiply in Tm-1/+ protoplasts to a greater extent with less delay when a large amount of inoculum RNA was used. However, viral production was completely blocked in Tm-1/Tm-1 protoplasts even when a large amount of L-RNA was used for inoculation.
Tm-1是一种使番茄对烟草花叶病毒（TMV）感染产生抗性的基因。为了研究这种抗性的生化机制，我们建立了三个番茄品系的细胞悬浮液，即 +/+（敏感，野生型，无Tm-1基因）、Tm-1/+（Tm-1基因杂合）和Tm-1/Tm-1（Tm-1基因纯合）。通过电穿孔法将TMV的番茄株系L和Lta1（最近从L中自发分离出的一个打破抗性的株系）的RNA接种到从这些细胞中分离出的原生质体上。在接种L的 +/+ 以及接种Lta1的 +/+、Tm-1/+、Tm-1/Tm-1原生质体中都能检测到所有病毒编码蛋白和TMV特异性RNA的合成，而在接种L的Tm-1/+原生质体中它们的产生明显减少。当使用大量接种物RNA时，L株系在Tm-1/+原生质体中能在更大程度上繁殖且延迟较少。然而，即使使用大量L-RNA进行接种，病毒在Tm-1/Tm-1原生质体中的产生也完全被阻断。
