Department of Physiology, Faculty of Natural Sciences of Szczecin University, Al. Piastów 40b, Szczecin, Poland.
J Physiol Biochem. 2010 Sep;66(3):189-96. doi: 10.1007/s13105-010-0023-9. Epub 2010 Jun 9.
During prolonged maximal exercise, oxygen deficits occur in working muscles. Progressive hypoxia results in the impairment of the oxidative resynthesis of ATP and increased degradation of purine nucleotides. Moreover, ATP consumption decreases the conversion of UDP to UTP, to use ATP as a phosphate donor, resulting in an increased concentration of UDP, which enhances pyrimidine degradation. Because the metabolism of pyrimidine nucleotides is related to the metabolism of purines, in particular with the cellular concentration of ATP, we decided to investigate the impact of a standardized exercise with increasing intensity on the concentration of uridine, inosine, hypoxanthine, and uric acid. Twenty-two healthy male subjects volunteered to participate in this study. Blood concentrations of metabolites were determined at rest, immediately after exercise, and after 30 min of recovery using high-performance liquid chromatography. We also studied the relationship between the levels of uridine and indicators of myogenic purine degradation. The results showed that exercise with increasing intensity leads to increased concentrations of inosine, hypoxanthine, uric acid, and uridine. We found positive correlations between blood uridine levels and indicators of myogenic purine degradation (hypoxanthine), suggesting that the blood uridine level is related to purine metabolism in skeletal muscles.
在长时间的最大运动中，工作肌肉会出现氧气不足。逐渐缺氧会导致 ATP 的氧化再合成受损，嘌呤核苷酸的降解增加。此外，ATP 的消耗会降低 UDP 向 UTP 的转化，以利用 ATP 作为磷酸供体，导致 UDP 浓度增加，从而增强嘧啶的降解。由于嘧啶核苷酸的代谢与嘌呤的代谢有关，特别是与细胞内 ATP 的浓度有关，我们决定研究标准化的递增强度运动对尿苷、肌苷、次黄嘌呤和尿酸浓度的影响。22 名健康男性志愿者自愿参加了这项研究。使用高效液相色谱法在休息时、运动后立即和恢复 30 分钟后测定代谢物的血液浓度。我们还研究了尿苷水平与肌源性嘌呤降解指标之间的关系。结果表明，强度递增的运动导致肌苷、次黄嘌呤、尿酸和尿苷浓度升高。我们发现血液尿苷水平与肌源性嘌呤降解指标（次黄嘌呤）之间存在正相关关系，表明血液尿苷水平与骨骼肌中的嘌呤代谢有关。
