Department of Pediatrics, Kansai Medical University, Moriguchi-shi, Osaka, Japan.
Int J Vitam Nutr Res. 2009 Sep;79(5-6):375-80. doi: 10.1024/0300-9831.79.56.375.
Although calcium is an essential mineral for bones, little is known about its effect on inflammatory or oxidative regulation. We hypothesize that calcium plays the role in the production of reactive oxygen species (ROS) and nitric oxide (NO) from polymorphonuclear leukocytes (PMNs). Our purpose was to determine the relationship of NO and ROS produced from PMNs, taking into account the role of calcium and magnesium in diet. Using flow cytometry, we compared ROS and NO production from PMNs after the stimulation by S. aureus or phorbol myristate acetate (PMA). PMNs taken from three murine groups were analyzed: C57BL/6 wild-type mice, low-calcium diet (Low Ca Group), or low-magnesium diet fed for 2 weeks. ROS production at baseline in the Low Ca Group was highest among the groups. PMA- and S. aureus-stimulated ROS production was also highest in the Low Ca Group. On the contrary, NO production at baseline in the Low Ca Group was lowest among the groups, while there was no significant difference among the groups in S. aureus-stimulated PMNs. A low-calcium diet increases ROS production from stimulated PMNs and decreases NO production at baseline. This finding suggests that calcium regulates ROS production from PMNs.
尽管钙是骨骼必需的矿物质，但人们对其在炎症或氧化调节中的作用知之甚少。我们假设钙在多形核白细胞（PMN）产生活性氧（ROS）和一氧化氮（NO）中发挥作用。我们的目的是确定从 PMN 产生的 NO 和 ROS 之间的关系，同时考虑钙和镁在饮食中的作用。我们使用流式细胞术比较了金黄色葡萄球菌或佛波酯（PMA）刺激后 PMN 产生的 ROS 和 NO。分析了来自三组小鼠的 PMN：C57BL/6 野生型小鼠、低钙饮食（Low Ca 组）或低镁饮食喂养 2 周的小鼠。在低钙组中，基础状态下的 ROS 产生量在所有组中最高。在低钙组中，PMA 和金黄色葡萄球菌刺激的 ROS 产生也最高。相反，在低钙组中，基础状态下的 NO 产生量在所有组中最低，而金黄色葡萄球菌刺激的 PMN 之间没有显著差异。低钙饮食会增加刺激 PMN 产生的 ROS 产生，并降低基础状态下的 NO 产生。这一发现表明钙调节 PMN 产生的 ROS。
