School of Chemistry and Chemical Engineering, Central South University, Changsha, P. R. China.
J Sep Sci. 2010 Jul;33(14):2160-7. doi: 10.1002/jssc.201000085.
The purpose of the research was to investigate the multiple response optimizations for the extraction of protopine and allocryptopine from the stems of Macleaya cordata (Willd) R. Br. by using microwave-assisted extraction (MAE). A three-level, three-factor Box-Behnken design of response surface methodology was used to develop response model, and desirability function was employed to optimize the effects of main extraction parameters. Three variables, ethanol concentration (20-80%, v/v), extraction temperature (30-70 degrees C) and solvent/solid ratio (10:1 to 30:1, mL/g), were investigated in this study. The results showed that the optimum parameters of MAE were ethanol concentration of 45.2 % (v/v), extraction temperature of 54.7 degrees C and solvent/solid ratio of 20.4:1 (mL/g). Under these conditions, the extraction yields of protopine and allocryptopine were 89.4 and 102.0%, respectively, and the extracta sicca yield was 12.5%. The combination use of response surface methodology, Box-Behnken design and the appropriate desirability function could provide an insight into a lab-scale MAE process, and help to develop procedures for commercial production of active ingredients from medical plants.
本研究旨在利用微波辅助提取（MAE）对博落回茎中普罗托品和阿朴可托品进行多响应优化提取。采用三水平三因素 Box-Behnken 设计响应面法建立响应模型，并采用适宜性函数对主要提取参数的影响进行优化。本研究考察了三个变量，即乙醇浓度（20-80%，v/v）、提取温度（30-70°C）和溶剂/固比（10:1 至 30:1，mL/g）。结果表明，MAE 的最佳参数为乙醇浓度 45.2%（v/v）、提取温度 54.7°C 和溶剂/固比 20.4:1（mL/g）。在此条件下，普罗托品和阿朴可托品的提取率分别为 89.4%和 102.0%，干燥提取物得率为 12.5%。响应面法、Box-Behnken 设计和适宜性函数的组合使用可以深入了解实验室规模的 MAE 工艺，并有助于开发从药用植物中提取活性成分的商业生产工艺。
