Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, P. R. China.
J Sep Sci. 2010 Jul;33(13):2026-34. doi: 10.1002/jssc.201000103.
A technique based on strip dispersion hybrid liquid membrane was developed for the separation and extraction of four main alkaloids from fruits of Macleaya cordata (Willd) R. Br. A microporous polypropylene membrane impregnated with an organic membrane solution comprised the heart of the strip dispersion hybrid liquid membrane system. The membrane solution was made by dissolving a cationic carrier, di-(2-ethylhexyl) phosphoric acid in an inexpensive, less toxic membrane solvent, kerosene. The transport of alkaloids from an aqueous feed solution through the membrane to a strip dispersion phase was driven by the concentration gradient of H(+) and facilitated by di-(2-ethylhexyl) phosphoric acid. The effects of the extraction time and reuse times of the membrane, the strip solution composition, the carrier concentration, the volume ratio of the aqueous strip solution to the organic membrane solution, and the flow rates of the feed solution and the strip dispersion phase on the transport of alkaloids were investigated. Under the optimal conditions, the permeability coefficients obtained for the four main alkaloids allocryptopine, protopine, sanguinarine, and chelerythrine were 1.66, 1.99, 2.98, and 3.06 x 10(-4) cm/s, and the transport efficiencies were as high as 68, 77, 83, and 85%, respectively.
基于条带分散混合液膜技术，从博落回果实中分离提取四种主要生物碱。微孔聚丙烯膜浸渍有机膜溶液是条带分散混合液膜系统的核心。膜溶液由阳离子载体二-(2-乙基己基)磷酸溶解在廉价、低毒的膜溶剂煤油中制成。在 H(+)浓度梯度的驱动下，通过膜将生物碱从水进料溶液中传输到条带分散相中，并通过二-(2-乙基己基)磷酸促进传输。考察了萃取时间和膜的重复使用次数、条带溶液组成、载体浓度、水条带溶液与有机膜溶液的体积比以及进料溶液和条带分散相的流速对生物碱传输的影响。在最佳条件下，四种主要生物碱阿朴啡、原阿朴啡、血根碱和白屈菜红碱的渗透率分别为 1.66、1.99、2.98 和 3.06×10(-4)cm/s，传输效率分别高达 68%、77%、83%和 85%。
